Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25495 and BPHYT_RS25500 are separated by 158 nucleotides BPHYT_RS25500 and BPHYT_RS25505 overlap by 4 nucleotides BPHYT_RS25505 and BPHYT_RS25510 are separated by 16 nucleotides BPHYT_RS25510 and BPHYT_RS25515 are separated by 83 nucleotides
BPHYT_RS25495: BPHYT_RS25495 - membrane protein, at 1,296,696 to 1,297,007
_RS25495
BPHYT_RS25500: BPHYT_RS25500 - hypothetical protein, at 1,297,166 to 1,297,807
_RS25500
BPHYT_RS25505: BPHYT_RS25505 - RNA polymerase sigma factor, at 1,297,804 to 1,298,349
_RS25505
BPHYT_RS25510: BPHYT_RS25510 - hypothetical protein, at 1,298,366 to 1,298,686
_RS25510
BPHYT_RS25515: BPHYT_RS25515 - glutathione S-transferase, at 1,298,770 to 1,299,471
_RS25515
Position (kb)
1297
1298
1299 Strain fitness (log2 ratio)
-1
0
1 at 1297.045 kb on + strand at 1297.046 kb on - strand at 1297.054 kb on + strand at 1297.222 kb on + strand at 1297.234 kb on - strand, within BPHYT_RS25500 at 1297.509 kb on - strand, within BPHYT_RS25500 at 1297.544 kb on - strand, within BPHYT_RS25500 at 1298.100 kb on + strand, within BPHYT_RS25505 at 1298.100 kb on + strand, within BPHYT_RS25505 at 1299.243 kb on - strand, within BPHYT_RS25515
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 1,297,045 + +0.2 1,297,046 - -0.0 1,297,054 + -0.3 1,297,222 + -1.3 1,297,234 - BPHYT_RS25500 0.11 -0.5 1,297,509 - BPHYT_RS25500 0.53 -0.5 1,297,544 - BPHYT_RS25500 0.59 -0.6 1,298,100 + BPHYT_RS25505 0.54 -0.5 1,298,100 + BPHYT_RS25505 0.54 -1.0 1,299,243 - BPHYT_RS25515 0.67 -0.2
Or see this region's nucleotide sequence