Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25480 and BPHYT_RS25485 are separated by 91 nucleotides BPHYT_RS25485 and BPHYT_RS25490 overlap by 11 nucleotides BPHYT_RS25490 and BPHYT_RS25495 are separated by 32 nucleotides BPHYT_RS25495 and BPHYT_RS25500 are separated by 158 nucleotides
BPHYT_RS25480: BPHYT_RS25480 - hypothetical protein, at 1,294,238 to 1,294,879
_RS25480
BPHYT_RS25485: BPHYT_RS25485 - hypothetical protein, at 1,294,971 to 1,295,750
_RS25485
BPHYT_RS25490: BPHYT_RS25490 - hypothetical protein, at 1,295,740 to 1,296,663
_RS25490
BPHYT_RS25495: BPHYT_RS25495 - membrane protein, at 1,296,696 to 1,297,007
_RS25495
BPHYT_RS25500: BPHYT_RS25500 - hypothetical protein, at 1,297,166 to 1,297,807
_RS25500
Position (kb)
1295
1296
1297 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1295.848 kb on - strand, within BPHYT_RS25490 at 1295.982 kb on + strand, within BPHYT_RS25490 at 1296.035 kb on + strand, within BPHYT_RS25490 at 1296.084 kb on + strand, within BPHYT_RS25490 at 1296.495 kb on + strand, within BPHYT_RS25490 at 1296.498 kb on - strand, within BPHYT_RS25490 at 1296.654 kb on + strand at 1297.045 kb on + strand at 1297.046 kb on - strand at 1297.054 kb on + strand at 1297.222 kb on + strand at 1297.234 kb on - strand, within BPHYT_RS25500 at 1297.509 kb on - strand, within BPHYT_RS25500 at 1297.544 kb on - strand, within BPHYT_RS25500
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 1,295,848 - BPHYT_RS25490 0.12 -0.8 1,295,982 + BPHYT_RS25490 0.26 -1.5 1,296,035 + BPHYT_RS25490 0.32 -0.5 1,296,084 + BPHYT_RS25490 0.37 +0.0 1,296,495 + BPHYT_RS25490 0.82 -0.6 1,296,498 - BPHYT_RS25490 0.82 -0.8 1,296,654 + -2.8 1,297,045 + +0.2 1,297,046 - -0.0 1,297,054 + -0.3 1,297,222 + -1.3 1,297,234 - BPHYT_RS25500 0.11 -0.5 1,297,509 - BPHYT_RS25500 0.53 -0.5 1,297,544 - BPHYT_RS25500 0.59 -0.6
Or see this region's nucleotide sequence