Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25000 and BPHYT_RS25005 are separated by 38 nucleotides BPHYT_RS25005 and BPHYT_RS25010 are separated by 194 nucleotides BPHYT_RS25010 and BPHYT_RS25015 are separated by 239 nucleotides BPHYT_RS25015 and BPHYT_RS25020 are separated by 138 nucleotides
BPHYT_RS25000: BPHYT_RS25000 - MFS transporter, at 1,178,594 to 1,179,838
_RS25000
BPHYT_RS25005: BPHYT_RS25005 - FMN reductase, at 1,179,877 to 1,180,422
_RS25005
BPHYT_RS25010: BPHYT_RS25010 - N5,N10-methylene tetrahydromethanopterin reductase, at 1,180,617 to 1,182,008
_RS25010
BPHYT_RS25015: BPHYT_RS25015 - XRE family transcriptional regulator, at 1,182,248 to 1,182,817
_RS25015
BPHYT_RS25020: BPHYT_RS25020 - phospholipid-binding protein, at 1,182,956 to 1,183,315
_RS25020
Position (kb)
1180
1181
1182
1183 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1180.002 kb on + strand, within BPHYT_RS25005 at 1180.003 kb on - strand, within BPHYT_RS25005 at 1180.747 kb on + strand at 1180.818 kb on - strand, within BPHYT_RS25010 at 1180.849 kb on - strand, within BPHYT_RS25010 at 1180.849 kb on - strand, within BPHYT_RS25010 at 1181.336 kb on - strand, within BPHYT_RS25010 at 1181.441 kb on - strand, within BPHYT_RS25010 at 1181.503 kb on + strand, within BPHYT_RS25010 at 1181.504 kb on - strand, within BPHYT_RS25010 at 1181.519 kb on - strand, within BPHYT_RS25010 at 1181.672 kb on - strand, within BPHYT_RS25010 at 1181.672 kb on - strand, within BPHYT_RS25010 at 1181.897 kb on - strand at 1181.901 kb on + strand at 1181.909 kb on + strand at 1181.910 kb on - strand at 1182.097 kb on - strand at 1182.201 kb on - strand at 1182.513 kb on + strand, within BPHYT_RS25015 at 1182.513 kb on + strand, within BPHYT_RS25015 at 1182.707 kb on + strand, within BPHYT_RS25015 at 1182.815 kb on + strand at 1182.815 kb on + strand at 1182.829 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 1,180,002 + BPHYT_RS25005 0.23 -0.5 1,180,003 - BPHYT_RS25005 0.23 +0.8 1,180,747 + +2.5 1,180,818 - BPHYT_RS25010 0.14 +0.3 1,180,849 - BPHYT_RS25010 0.17 +0.7 1,180,849 - BPHYT_RS25010 0.17 -0.3 1,181,336 - BPHYT_RS25010 0.52 -0.2 1,181,441 - BPHYT_RS25010 0.59 -0.7 1,181,503 + BPHYT_RS25010 0.64 -0.4 1,181,504 - BPHYT_RS25010 0.64 -1.3 1,181,519 - BPHYT_RS25010 0.65 +0.2 1,181,672 - BPHYT_RS25010 0.76 -1.2 1,181,672 - BPHYT_RS25010 0.76 -3.2 1,181,897 - -0.8 1,181,901 + +0.7 1,181,909 + -1.1 1,181,910 - -0.8 1,182,097 - +0.7 1,182,201 - -0.4 1,182,513 + BPHYT_RS25015 0.46 -1.7 1,182,513 + BPHYT_RS25015 0.46 +1.5 1,182,707 + BPHYT_RS25015 0.81 -2.3 1,182,815 + -0.1 1,182,815 + +0.0 1,182,829 + +1.3
Or see this region's nucleotide sequence