Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS23460 and BPHYT_RS23465 are separated by 213 nucleotides BPHYT_RS23465 and BPHYT_RS23470 are separated by 89 nucleotides BPHYT_RS23470 and BPHYT_RS23475 are separated by 72 nucleotides
BPHYT_RS23460: BPHYT_RS23460 - AraC family transcriptional regulator, at 831,153 to 832,109
_RS23460
BPHYT_RS23465: BPHYT_RS23465 - aliphatic nitrilase, at 832,323 to 833,309
_RS23465
BPHYT_RS23470: BPHYT_RS23470 - carbonic anhydrase, at 833,399 to 833,953
_RS23470
BPHYT_RS23475: BPHYT_RS23475 - luciferase, at 834,026 to 835,033
_RS23475
Position (kb) 832
833
834 Strain fitness (log2 ratio) -1
0
1
2 at 832.110 kb on - strand at 832.110 kb on - strand at 832.253 kb on + strand at 832.371 kb on + strand at 832.372 kb on - strand at 832.470 kb on + strand, within BPHYT_RS23465 at 832.471 kb on - strand, within BPHYT_RS23465 at 832.471 kb on - strand, within BPHYT_RS23465 at 832.648 kb on - strand, within BPHYT_RS23465 at 833.015 kb on + strand, within BPHYT_RS23465 at 833.085 kb on + strand, within BPHYT_RS23465 at 833.085 kb on + strand, within BPHYT_RS23465 at 833.447 kb on - strand at 833.488 kb on + strand, within BPHYT_RS23470 at 833.714 kb on - strand, within BPHYT_RS23470 at 834.084 kb on + strand at 834.177 kb on - strand, within BPHYT_RS23475 at 834.202 kb on + strand, within BPHYT_RS23475
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 832,110 - +0.9 832,110 - -0.5 832,253 + +0.3 832,371 + -0.3 832,372 - +0.8 832,470 + BPHYT_RS23465 0.15 -0.4 832,471 - BPHYT_RS23465 0.15 +1.3 832,471 - BPHYT_RS23465 0.15 +0.6 832,648 - BPHYT_RS23465 0.33 -1.0 833,015 + BPHYT_RS23465 0.70 +1.5 833,085 + BPHYT_RS23465 0.77 +2.6 833,085 + BPHYT_RS23465 0.77 +0.9 833,447 - +0.8 833,488 + BPHYT_RS23470 0.16 -1.4 833,714 - BPHYT_RS23470 0.57 +0.6 834,084 + +0.8 834,177 - BPHYT_RS23475 0.15 -0.2 834,202 + BPHYT_RS23475 0.17 +0.0
Or see this region's nucleotide sequence