Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22720 and BPHYT_RS22725 are separated by 195 nucleotides BPHYT_RS22725 and BPHYT_RS22730 are separated by 52 nucleotides
BPHYT_RS22720: BPHYT_RS22720 - LysR family transcriptional regulator, at 652,473 to 653,456
_RS22720
BPHYT_RS22725: BPHYT_RS22725 - glycosyl transferase family 51, at 653,652 to 656,732
_RS22725
BPHYT_RS22730: BPHYT_RS22730 - serine/threonine kinase, at 656,785 to 658,011
_RS22730
Position (kb) 653
654
655
656
657 Strain fitness (log2 ratio) -1
0
1
2 at 652.760 kb on - strand, within BPHYT_RS22720 at 652.974 kb on + strand, within BPHYT_RS22720 at 652.975 kb on - strand, within BPHYT_RS22720 at 652.981 kb on - strand, within BPHYT_RS22720 at 653.091 kb on + strand, within BPHYT_RS22720 at 653.593 kb on - strand at 653.812 kb on - strand at 654.537 kb on + strand, within BPHYT_RS22725 at 654.664 kb on - strand, within BPHYT_RS22725 at 654.682 kb on - strand, within BPHYT_RS22725 at 654.682 kb on - strand, within BPHYT_RS22725 at 654.711 kb on + strand, within BPHYT_RS22725 at 655.204 kb on - strand, within BPHYT_RS22725 at 655.851 kb on - strand, within BPHYT_RS22725 at 655.858 kb on - strand, within BPHYT_RS22725 at 655.904 kb on + strand, within BPHYT_RS22725 at 656.601 kb on + strand at 656.711 kb on + strand at 656.846 kb on + strand at 656.847 kb on - strand at 657.527 kb on - strand, within BPHYT_RS22730
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 652,760 - BPHYT_RS22720 0.29 +2.1 652,974 + BPHYT_RS22720 0.51 +1.4 652,975 - BPHYT_RS22720 0.51 +0.4 652,981 - BPHYT_RS22720 0.52 -0.4 653,091 + BPHYT_RS22720 0.63 -1.6 653,593 - -0.4 653,812 - -1.3 654,537 + BPHYT_RS22725 0.29 +0.5 654,664 - BPHYT_RS22725 0.33 +1.4 654,682 - BPHYT_RS22725 0.33 +1.2 654,682 - BPHYT_RS22725 0.33 -1.0 654,711 + BPHYT_RS22725 0.34 +1.6 655,204 - BPHYT_RS22725 0.50 -0.3 655,851 - BPHYT_RS22725 0.71 +0.2 655,858 - BPHYT_RS22725 0.72 -0.2 655,904 + BPHYT_RS22725 0.73 +1.0 656,601 + +1.0 656,711 + +1.5 656,846 + +0.6 656,847 - -0.8 657,527 - BPHYT_RS22730 0.60 +1.0
Or see this region's nucleotide sequence