Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22390 and BPHYT_RS22395 are separated by 347 nucleotides BPHYT_RS22395 and BPHYT_RS22400 are separated by 181 nucleotides BPHYT_RS22400 and BPHYT_RS22405 are separated by 57 nucleotides
BPHYT_RS22390: BPHYT_RS22390 - glycine/betaine ABC transporter substrate-binding protein, at 579,184 to 580,149
_RS22390
BPHYT_RS22395: BPHYT_RS22395 - AraC family transcriptional regulator, at 580,497 to 581,450
_RS22395
BPHYT_RS22400: BPHYT_RS22400 - hypothetical protein, at 581,632 to 581,958
_RS22400
BPHYT_RS22405: BPHYT_RS22405 - HxlR family transcriptional regulator, at 582,016 to 582,417
_RS22405
Position (kb) 580
581
582 Strain fitness (log2 ratio) -2
-1
0
1 at 579.689 kb on + strand, within BPHYT_RS22390 at 579.762 kb on - strand, within BPHYT_RS22390 at 579.802 kb on - strand, within BPHYT_RS22390 at 580.282 kb on - strand at 580.391 kb on + strand at 580.391 kb on + strand at 580.508 kb on + strand at 580.508 kb on + strand at 580.743 kb on + strand, within BPHYT_RS22395 at 580.743 kb on + strand, within BPHYT_RS22395 at 580.958 kb on + strand, within BPHYT_RS22395 at 581.275 kb on - strand, within BPHYT_RS22395 at 581.547 kb on - strand at 581.548 kb on + strand at 581.552 kb on + strand at 581.553 kb on - strand at 581.553 kb on - strand at 581.562 kb on + strand at 581.794 kb on + strand, within BPHYT_RS22400 at 581.926 kb on + strand at 581.944 kb on - strand at 581.975 kb on + strand at 581.975 kb on + strand at 581.976 kb on - strand at 581.979 kb on - strand at 582.006 kb on - strand at 582.017 kb on + strand at 582.417 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 579,689 + BPHYT_RS22390 0.52 -2.0 579,762 - BPHYT_RS22390 0.60 +0.7 579,802 - BPHYT_RS22390 0.64 -1.9 580,282 - -0.1 580,391 + +0.1 580,391 + -2.1 580,508 + +0.7 580,508 + +1.4 580,743 + BPHYT_RS22395 0.26 -0.4 580,743 + BPHYT_RS22395 0.26 +1.5 580,958 + BPHYT_RS22395 0.48 +0.4 581,275 - BPHYT_RS22395 0.82 +0.3 581,547 - -0.8 581,548 + -2.1 581,552 + -1.2 581,553 - -0.1 581,553 - -0.1 581,562 + -0.2 581,794 + BPHYT_RS22400 0.50 -0.5 581,926 + -0.6 581,944 - -0.4 581,975 + -0.3 581,975 + +0.7 581,976 - -0.5 581,979 - -0.7 582,006 - +0.7 582,017 + -0.5 582,417 + -0.1
Or see this region's nucleotide sequence