Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20855 and BPHYT_RS20860 are separated by 204 nucleotides BPHYT_RS20860 and BPHYT_RS20865 are separated by 33 nucleotides BPHYT_RS20865 and BPHYT_RS20870 are separated by 688 nucleotides
BPHYT_RS20855: BPHYT_RS20855 - transcriptional regulator, at 241,250 to 242,158
_RS20855
BPHYT_RS20860: BPHYT_RS20860 - hypothetical protein, at 242,363 to 242,686
_RS20860
BPHYT_RS20865: BPHYT_RS20865 - 2-hydroxyacid dehydrogenase, at 242,720 to 243,685
_RS20865
BPHYT_RS20870: BPHYT_RS20870 - cysteine hydrolase, at 244,374 to 244,943
_RS20870
Position (kb) 242
243
244 Strain fitness (log2 ratio) -1
0
1 at 241.829 kb on - strand, within BPHYT_RS20855 at 241.953 kb on + strand, within BPHYT_RS20855 at 241.954 kb on - strand, within BPHYT_RS20855 at 242.060 kb on + strand, within BPHYT_RS20855 at 242.061 kb on - strand, within BPHYT_RS20855 at 242.128 kb on + strand at 242.229 kb on + strand at 242.785 kb on - strand at 243.424 kb on + strand, within BPHYT_RS20865 at 243.424 kb on + strand, within BPHYT_RS20865 at 243.916 kb on + strand at 244.052 kb on + strand at 244.375 kb on + strand at 244.444 kb on - strand, within BPHYT_RS20870 at 244.475 kb on - strand, within BPHYT_RS20870 at 244.475 kb on - strand, within BPHYT_RS20870
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 241,829 - BPHYT_RS20855 0.64 +1.5 241,953 + BPHYT_RS20855 0.77 -0.2 241,954 - BPHYT_RS20855 0.77 -0.8 242,060 + BPHYT_RS20855 0.89 +0.5 242,061 - BPHYT_RS20855 0.89 +0.1 242,128 + +0.3 242,229 + -0.8 242,785 - -0.2 243,424 + BPHYT_RS20865 0.73 -1.1 243,424 + BPHYT_RS20865 0.73 -0.6 243,916 + -0.4 244,052 + +0.2 244,375 + +0.9 244,444 - BPHYT_RS20870 0.12 -1.0 244,475 - BPHYT_RS20870 0.18 -1.2 244,475 - BPHYT_RS20870 0.18 -0.4
Or see this region's nucleotide sequence