Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS20105

Experiment: 36hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS20100 and BPHYT_RS20105 are separated by 155 nucleotidesBPHYT_RS20105 and BPHYT_RS20110 are separated by 66 nucleotides BPHYT_RS20100: BPHYT_RS20100 - sensor histidine kinase, at 51,829 to 53,223 _RS20100 BPHYT_RS20105: BPHYT_RS20105 - nitrate ABC transporter substrate-binding protein, at 53,379 to 54,419 _RS20105 BPHYT_RS20110: BPHYT_RS20110 - sensor histidine kinase, at 54,486 to 55,868 _RS20110 Position (kb) 53 54 55Strain fitness (log2 ratio) -1 0 1 2at 52.683 kb on + strand, within BPHYT_RS20100at 52.930 kb on - strand, within BPHYT_RS20100at 53.211 kb on + strandat 53.895 kb on + strand, within BPHYT_RS20105at 53.896 kb on - strand, within BPHYT_RS20105at 54.035 kb on + strand, within BPHYT_RS20105at 54.036 kb on - strand, within BPHYT_RS20105at 54.421 kb on - strandat 54.738 kb on + strand, within BPHYT_RS20110at 54.979 kb on + strand, within BPHYT_RS20110at 55.023 kb on + strand, within BPHYT_RS20110

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1
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52,683 + BPHYT_RS20100 0.61 +0.6
52,930 - BPHYT_RS20100 0.79 +0.4
53,211 + -0.8
53,895 + BPHYT_RS20105 0.50 +2.6
53,896 - BPHYT_RS20105 0.50 +0.3
54,035 + BPHYT_RS20105 0.63 -0.3
54,036 - BPHYT_RS20105 0.63 -0.2
54,421 - +0.0
54,738 + BPHYT_RS20110 0.18 +2.6
54,979 + BPHYT_RS20110 0.36 +0.5
55,023 + BPHYT_RS20110 0.39 -0.1

Or see this region's nucleotide sequence