Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19945 and BPHYT_RS19950 are separated by 67 nucleotides BPHYT_RS19950 and BPHYT_RS19955 overlap by 4 nucleotides BPHYT_RS19955 and BPHYT_RS19960 are separated by 100 nucleotides
BPHYT_RS19945: BPHYT_RS19945 - hypothetical protein, at 20,520 to 21,017
_RS19945
BPHYT_RS19950: BPHYT_RS19950 - hypothetical protein, at 21,085 to 21,342
_RS19950
BPHYT_RS19955: BPHYT_RS19955 - LysR family transcriptional regulator, at 21,339 to 22,217
_RS19955
BPHYT_RS19960: BPHYT_RS19960 - class A beta-lactamase, at 22,318 to 23,265
_RS19960
Position (kb)
21
22 Strain fitness (log2 ratio)
-2
-1
0
1 at 20.106 kb on + strand at 20.107 kb on - strand at 20.120 kb on - strand at 20.120 kb on - strand at 20.643 kb on + strand, within BPHYT_RS19945 at 20.670 kb on + strand, within BPHYT_RS19945 at 20.778 kb on + strand, within BPHYT_RS19945 at 21.200 kb on - strand, within BPHYT_RS19950 at 21.203 kb on + strand, within BPHYT_RS19950 at 21.203 kb on + strand, within BPHYT_RS19950 at 21.203 kb on + strand, within BPHYT_RS19950 at 21.203 kb on + strand, within BPHYT_RS19950 at 21.204 kb on - strand, within BPHYT_RS19950 at 21.204 kb on - strand, within BPHYT_RS19950 at 21.204 kb on - strand, within BPHYT_RS19950 at 22.053 kb on + strand, within BPHYT_RS19955 at 22.089 kb on + strand, within BPHYT_RS19955 at 22.090 kb on - strand, within BPHYT_RS19955 at 22.255 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 20,106 + -0.2 20,107 - +0.1 20,120 - -2.4 20,120 - -0.1 20,643 + BPHYT_RS19945 0.25 -1.8 20,670 + BPHYT_RS19945 0.30 -0.7 20,778 + BPHYT_RS19945 0.52 -0.4 21,200 - BPHYT_RS19950 0.45 -1.9 21,203 + BPHYT_RS19950 0.46 +0.1 21,203 + BPHYT_RS19950 0.46 +0.1 21,203 + BPHYT_RS19950 0.46 -1.4 21,203 + BPHYT_RS19950 0.46 +0.3 21,204 - BPHYT_RS19950 0.46 +0.3 21,204 - BPHYT_RS19950 0.46 +0.8 21,204 - BPHYT_RS19950 0.46 +0.5 22,053 + BPHYT_RS19955 0.81 +0.2 22,089 + BPHYT_RS19955 0.85 -0.8 22,090 - BPHYT_RS19955 0.85 +0.1 22,255 + +0.3
Or see this region's nucleotide sequence