Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19885 and BPHYT_RS19890 are separated by 368 nucleotides BPHYT_RS19890 and BPHYT_RS19895 are separated by 47 nucleotides BPHYT_RS19895 and BPHYT_RS19900 are separated by 303 nucleotides BPHYT_RS19900 and BPHYT_RS19905 are separated by 279 nucleotides
BPHYT_RS19885: BPHYT_RS19885 - hypothetical protein, at 3,938 to 5,911
_RS19885
BPHYT_RS19890: BPHYT_RS19890 - hypothetical protein, at 6,280 to 6,783
_RS19890
BPHYT_RS19895: BPHYT_RS19895 - rod shape-determining protein RodA, at 6,831 to 7,265
_RS19895
BPHYT_RS19900: BPHYT_RS19900 - hypothetical protein, at 7,569 to 7,850
_RS19900
BPHYT_RS19905: BPHYT_RS19905 - membrane protein, at 8,130 to 9,530
_RS19905
Position (kb) 6
7
8 Strain fitness (log2 ratio) -1
0
1 at 5.943 kb on - strand at 6.035 kb on - strand at 6.038 kb on - strand at 6.112 kb on + strand at 6.113 kb on - strand at 6.128 kb on + strand at 6.129 kb on - strand at 6.129 kb on - strand at 6.129 kb on - strand at 6.379 kb on - strand, within BPHYT_RS19890 at 6.939 kb on - strand, within BPHYT_RS19895 at 7.653 kb on + strand, within BPHYT_RS19900
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 5,943 - -0.9 6,035 - +0.6 6,038 - +0.4 6,112 + -0.3 6,113 - +0.0 6,128 + -0.5 6,129 - -0.3 6,129 - +0.6 6,129 - -1.1 6,379 - BPHYT_RS19890 0.20 -1.4 6,939 - BPHYT_RS19895 0.25 +0.8 7,653 + BPHYT_RS19900 0.30 -1.2
Or see this region's nucleotide sequence