Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16130 and BPHYT_RS16135 overlap by 4 nucleotides BPHYT_RS16135 and BPHYT_RS16140 overlap by 8 nucleotides BPHYT_RS16140 and BPHYT_RS16145 are separated by 58 nucleotides BPHYT_RS16145 and BPHYT_RS16150 are separated by 14 nucleotides
BPHYT_RS16130: BPHYT_RS16130 - cation ABC transporter substrate-binding protein, at 3,658,958 to 3,659,926
_RS16130
BPHYT_RS16135: BPHYT_RS16135 - ABC transporter ATP-binding protein, at 3,659,923 to 3,660,864
_RS16135
BPHYT_RS16140: BPHYT_RS16140 - ABC transporter permease, at 3,660,857 to 3,661,639
_RS16140
BPHYT_RS16145: BPHYT_RS16145 - S-formylglutathione hydrolase, at 3,661,698 to 3,662,549
_RS16145
BPHYT_RS16150: BPHYT_RS16150 - alcohol dehydrogenase, at 3,662,564 to 3,663,670
_RS16150
Position (kb)
3660
3661
3662 Strain fitness (log2 ratio)
-2
-1
0
1 at 3660.194 kb on - strand, within BPHYT_RS16135 at 3660.195 kb on + strand, within BPHYT_RS16135 at 3660.196 kb on - strand, within BPHYT_RS16135 at 3660.377 kb on + strand, within BPHYT_RS16135 at 3660.378 kb on - strand, within BPHYT_RS16135 at 3660.593 kb on - strand, within BPHYT_RS16135 at 3660.593 kb on - strand, within BPHYT_RS16135 at 3660.593 kb on - strand, within BPHYT_RS16135 at 3660.635 kb on + strand, within BPHYT_RS16135 at 3660.636 kb on - strand, within BPHYT_RS16135 at 3661.229 kb on - strand, within BPHYT_RS16140 at 3661.559 kb on + strand, within BPHYT_RS16140 at 3661.674 kb on - strand at 3661.759 kb on + strand at 3661.759 kb on + strand at 3661.760 kb on - strand at 3661.760 kb on - strand at 3661.763 kb on - strand at 3661.774 kb on + strand at 3661.942 kb on + strand, within BPHYT_RS16145 at 3661.942 kb on + strand, within BPHYT_RS16145 at 3662.179 kb on + strand, within BPHYT_RS16145 at 3662.180 kb on - strand, within BPHYT_RS16145 at 3662.180 kb on - strand, within BPHYT_RS16145 at 3662.180 kb on - strand, within BPHYT_RS16145 at 3662.180 kb on - strand, within BPHYT_RS16145 at 3662.185 kb on + strand, within BPHYT_RS16145 at 3662.185 kb on + strand, within BPHYT_RS16145 at 3662.185 kb on + strand, within BPHYT_RS16145 at 3662.185 kb on + strand, within BPHYT_RS16145 at 3662.398 kb on + strand, within BPHYT_RS16145 at 3662.398 kb on + strand, within BPHYT_RS16145 at 3662.399 kb on - strand, within BPHYT_RS16145 at 3662.399 kb on - strand, within BPHYT_RS16145 at 3662.450 kb on - strand, within BPHYT_RS16145 at 3662.450 kb on - strand, within BPHYT_RS16145 at 3662.450 kb on - strand, within BPHYT_RS16145 at 3662.497 kb on + strand at 3662.498 kb on - strand at 3662.568 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 3,660,194 - BPHYT_RS16135 0.29 -0.0 3,660,195 + BPHYT_RS16135 0.29 -0.2 3,660,196 - BPHYT_RS16135 0.29 +0.2 3,660,377 + BPHYT_RS16135 0.48 -0.7 3,660,378 - BPHYT_RS16135 0.48 -0.8 3,660,593 - BPHYT_RS16135 0.71 -1.6 3,660,593 - BPHYT_RS16135 0.71 +0.2 3,660,593 - BPHYT_RS16135 0.71 -1.7 3,660,635 + BPHYT_RS16135 0.76 -0.4 3,660,636 - BPHYT_RS16135 0.76 +0.8 3,661,229 - BPHYT_RS16140 0.48 -0.7 3,661,559 + BPHYT_RS16140 0.90 -0.7 3,661,674 - -0.7 3,661,759 + +0.7 3,661,759 + -0.2 3,661,760 - -0.7 3,661,760 - -1.5 3,661,763 - -2.2 3,661,774 + +0.3 3,661,942 + BPHYT_RS16145 0.29 -0.1 3,661,942 + BPHYT_RS16145 0.29 -0.9 3,662,179 + BPHYT_RS16145 0.56 -0.5 3,662,180 - BPHYT_RS16145 0.57 -0.1 3,662,180 - BPHYT_RS16145 0.57 -1.1 3,662,180 - BPHYT_RS16145 0.57 +0.0 3,662,180 - BPHYT_RS16145 0.57 +0.0 3,662,185 + BPHYT_RS16145 0.57 +0.1 3,662,185 + BPHYT_RS16145 0.57 -1.2 3,662,185 + BPHYT_RS16145 0.57 +0.5 3,662,185 + BPHYT_RS16145 0.57 -0.4 3,662,398 + BPHYT_RS16145 0.82 -0.1 3,662,398 + BPHYT_RS16145 0.82 -0.1 3,662,399 - BPHYT_RS16145 0.82 -1.5 3,662,399 - BPHYT_RS16145 0.82 -0.3 3,662,450 - BPHYT_RS16145 0.88 -0.2 3,662,450 - BPHYT_RS16145 0.88 +0.2 3,662,450 - BPHYT_RS16145 0.88 -0.4 3,662,497 + -0.8 3,662,498 - +0.1 3,662,568 + -0.4
Or see this region's nucleotide sequence