Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15950 and BPHYT_RS15955 overlap by 4 nucleotides BPHYT_RS15955 and BPHYT_RS15960 are separated by 109 nucleotides BPHYT_RS15960 and BPHYT_RS15965 are separated by 17 nucleotides BPHYT_RS15965 and BPHYT_RS15970 overlap by 19 nucleotides
BPHYT_RS15950: BPHYT_RS15950 - tyrosine recombinase XerD, at 3,620,739 to 3,621,674
_RS15950
BPHYT_RS15955: BPHYT_RS15955 - methylated-DNA--protein-cysteine methyltransferase, at 3,621,671 to 3,622,141
_RS15955
BPHYT_RS15960: BPHYT_RS15960 - iron-sulfur cluster binding protein, at 3,622,251 to 3,623,489
_RS15960
BPHYT_RS15965: BPHYT_RS15965 - hypothetical protein, at 3,623,507 to 3,624,091
_RS15965
BPHYT_RS15970: BPHYT_RS15970 - N-acetylmuramoyl-L-alanine amidase, at 3,624,073 to 3,625,653
_RS15970
Position (kb)
3622
3623
3624 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3621.455 kb on - strand, within BPHYT_RS15950 at 3621.701 kb on + strand at 3621.873 kb on + strand, within BPHYT_RS15955 at 3621.873 kb on + strand, within BPHYT_RS15955 at 3621.874 kb on - strand, within BPHYT_RS15955 at 3621.988 kb on - strand, within BPHYT_RS15955 at 3622.062 kb on + strand, within BPHYT_RS15955 at 3622.190 kb on + strand at 3622.224 kb on - strand at 3622.247 kb on + strand at 3622.329 kb on + strand at 3622.330 kb on - strand at 3622.601 kb on - strand, within BPHYT_RS15960 at 3622.640 kb on + strand, within BPHYT_RS15960 at 3622.641 kb on - strand, within BPHYT_RS15960 at 3622.696 kb on + strand, within BPHYT_RS15960 at 3622.877 kb on + strand, within BPHYT_RS15960 at 3622.877 kb on + strand, within BPHYT_RS15960 at 3622.877 kb on + strand, within BPHYT_RS15960 at 3622.877 kb on + strand, within BPHYT_RS15960 at 3622.877 kb on + strand, within BPHYT_RS15960 at 3622.878 kb on - strand, within BPHYT_RS15960 at 3622.878 kb on - strand, within BPHYT_RS15960 at 3622.951 kb on - strand, within BPHYT_RS15960 at 3622.957 kb on + strand, within BPHYT_RS15960 at 3623.029 kb on + strand, within BPHYT_RS15960 at 3623.048 kb on - strand, within BPHYT_RS15960 at 3623.176 kb on + strand, within BPHYT_RS15960 at 3623.176 kb on + strand, within BPHYT_RS15960 at 3623.192 kb on + strand, within BPHYT_RS15960 at 3623.260 kb on + strand, within BPHYT_RS15960 at 3623.260 kb on + strand, within BPHYT_RS15960
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 3,621,455 - BPHYT_RS15950 0.76 -0.6 3,621,701 + -0.8 3,621,873 + BPHYT_RS15955 0.43 +0.2 3,621,873 + BPHYT_RS15955 0.43 -0.5 3,621,874 - BPHYT_RS15955 0.43 +0.9 3,621,988 - BPHYT_RS15955 0.67 +0.2 3,622,062 + BPHYT_RS15955 0.83 -0.8 3,622,190 + -0.1 3,622,224 - -0.2 3,622,247 + -1.0 3,622,329 + +0.1 3,622,330 - +0.4 3,622,601 - BPHYT_RS15960 0.28 +0.0 3,622,640 + BPHYT_RS15960 0.31 -0.2 3,622,641 - BPHYT_RS15960 0.31 +1.0 3,622,696 + BPHYT_RS15960 0.36 -0.1 3,622,877 + BPHYT_RS15960 0.51 -0.1 3,622,877 + BPHYT_RS15960 0.51 -0.4 3,622,877 + BPHYT_RS15960 0.51 -0.0 3,622,877 + BPHYT_RS15960 0.51 -0.4 3,622,877 + BPHYT_RS15960 0.51 -0.5 3,622,878 - BPHYT_RS15960 0.51 +2.9 3,622,878 - BPHYT_RS15960 0.51 +0.3 3,622,951 - BPHYT_RS15960 0.56 -0.7 3,622,957 + BPHYT_RS15960 0.57 -0.5 3,623,029 + BPHYT_RS15960 0.63 +0.8 3,623,048 - BPHYT_RS15960 0.64 +0.8 3,623,176 + BPHYT_RS15960 0.75 +0.2 3,623,176 + BPHYT_RS15960 0.75 +0.2 3,623,192 + BPHYT_RS15960 0.76 +0.5 3,623,260 + BPHYT_RS15960 0.81 +0.6 3,623,260 + BPHYT_RS15960 0.81 +1.2
Or see this region's nucleotide sequence