Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS15885 and BPHYT_RS15890 are separated by 271 nucleotides BPHYT_RS15890 and BPHYT_RS15895 are separated by 125 nucleotides BPHYT_RS15895 and BPHYT_RS15900 are separated by 5 nucleotides
BPHYT_RS15885: BPHYT_RS15885 - adenylate kinase, at 3,606,842 to 3,607,507
_RS15885
BPHYT_RS15890: BPHYT_RS15890 - 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, at 3,607,779 to 3,608,537
_RS15890
BPHYT_RS15895: BPHYT_RS15895 - transglutaminase, at 3,608,663 to 3,609,505
_RS15895
BPHYT_RS15900: BPHYT_RS15900 - membrane protein, at 3,609,511 to 3,611,061
_RS15900
Position (kb)
3607
3608
3609 Strain fitness (log2 ratio)
-2
-1
0
1 at 3606.820 kb on + strand at 3606.820 kb on + strand at 3606.824 kb on + strand at 3606.824 kb on + strand at 3607.505 kb on + strand at 3607.709 kb on + strand at 3607.710 kb on - strand at 3607.738 kb on - strand at 3607.753 kb on + strand at 3608.445 kb on + strand, within BPHYT_RS15890 at 3608.535 kb on + strand at 3608.535 kb on + strand at 3608.791 kb on - strand, within BPHYT_RS15895 at 3608.791 kb on - strand, within BPHYT_RS15895 at 3608.907 kb on + strand, within BPHYT_RS15895 at 3608.932 kb on + strand, within BPHYT_RS15895 at 3608.932 kb on + strand, within BPHYT_RS15895 at 3609.016 kb on - strand, within BPHYT_RS15895 at 3609.108 kb on - strand, within BPHYT_RS15895 at 3609.175 kb on - strand, within BPHYT_RS15895 at 3609.249 kb on + strand, within BPHYT_RS15895 at 3609.250 kb on - strand, within BPHYT_RS15895 at 3609.250 kb on - strand, within BPHYT_RS15895 at 3609.250 kb on - strand, within BPHYT_RS15895 at 3609.253 kb on - strand, within BPHYT_RS15895 at 3609.486 kb on + strand at 3609.486 kb on + strand at 3609.534 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 3,606,820 + +0.4 3,606,820 + -1.6 3,606,824 + -0.5 3,606,824 + -1.6 3,607,505 + -1.1 3,607,709 + -0.8 3,607,710 - +0.3 3,607,738 - -1.0 3,607,753 + -0.5 3,608,445 + BPHYT_RS15890 0.88 -1.2 3,608,535 + +0.4 3,608,535 + -0.2 3,608,791 - BPHYT_RS15895 0.15 -0.5 3,608,791 - BPHYT_RS15895 0.15 -1.2 3,608,907 + BPHYT_RS15895 0.29 -1.0 3,608,932 + BPHYT_RS15895 0.32 -0.6 3,608,932 + BPHYT_RS15895 0.32 -0.8 3,609,016 - BPHYT_RS15895 0.42 -2.0 3,609,108 - BPHYT_RS15895 0.53 -1.3 3,609,175 - BPHYT_RS15895 0.61 -1.0 3,609,249 + BPHYT_RS15895 0.70 -0.6 3,609,250 - BPHYT_RS15895 0.70 -0.2 3,609,250 - BPHYT_RS15895 0.70 -0.2 3,609,250 - BPHYT_RS15895 0.70 -0.4 3,609,253 - BPHYT_RS15895 0.70 -0.6 3,609,486 + -0.1 3,609,486 + -0.0 3,609,534 - +0.5
Or see this region's nucleotide sequence