Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13140 and BPHYT_RS13145 are separated by 28 nucleotides BPHYT_RS13145 and BPHYT_RS13150 are separated by 20 nucleotides BPHYT_RS13150 and BPHYT_RS13155 are separated by 291 nucleotides BPHYT_RS13155 and BPHYT_RS13160 are separated by 100 nucleotides
BPHYT_RS13140: BPHYT_RS13140 - MFS transporter, at 3,001,263 to 3,002,462
_RS13140
BPHYT_RS13145: BPHYT_RS13145 - chemotaxis protein CheY, at 3,002,491 to 3,002,919
_RS13145
BPHYT_RS13150: BPHYT_RS13150 - hisitidine kinase, at 3,002,940 to 3,003,572
_RS13150
BPHYT_RS13155: BPHYT_RS13155 - bacterioferritin, at 3,003,864 to 3,004,436
_RS13155
BPHYT_RS13160: BPHYT_RS13160 - membrane protein, at 3,004,537 to 3,004,698
_RS13160
Position (kb)
3002
3003
3004 Strain fitness (log2 ratio)
-1
0
1
2 at 3001.967 kb on + strand, within BPHYT_RS13140 at 3001.968 kb on - strand, within BPHYT_RS13140 at 3002.304 kb on + strand, within BPHYT_RS13140 at 3002.305 kb on - strand, within BPHYT_RS13140 at 3002.317 kb on - strand, within BPHYT_RS13140 at 3002.926 kb on - strand at 3002.971 kb on + strand at 3003.025 kb on + strand, within BPHYT_RS13150 at 3003.556 kb on - strand at 3003.626 kb on + strand at 3003.627 kb on - strand at 3003.627 kb on - strand at 3003.627 kb on - strand at 3003.821 kb on + strand at 3003.822 kb on - strand at 3003.865 kb on + strand at 3003.866 kb on - strand at 3003.866 kb on - strand at 3003.938 kb on + strand, within BPHYT_RS13155 at 3003.989 kb on - strand, within BPHYT_RS13155 at 3004.004 kb on + strand, within BPHYT_RS13155 at 3004.005 kb on - strand, within BPHYT_RS13155 at 3004.278 kb on - strand, within BPHYT_RS13155 at 3004.278 kb on - strand, within BPHYT_RS13155 at 3004.538 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 3,001,967 + BPHYT_RS13140 0.59 -1.5 3,001,968 - BPHYT_RS13140 0.59 +0.7 3,002,304 + BPHYT_RS13140 0.87 -0.5 3,002,305 - BPHYT_RS13140 0.87 +0.7 3,002,317 - BPHYT_RS13140 0.88 -0.8 3,002,926 - -0.7 3,002,971 + +1.4 3,003,025 + BPHYT_RS13150 0.13 +0.8 3,003,556 - +1.3 3,003,626 + -0.8 3,003,627 - +0.8 3,003,627 - +1.5 3,003,627 - +0.8 3,003,821 + +2.0 3,003,822 - +0.2 3,003,865 + -1.4 3,003,866 - -0.4 3,003,866 - +0.5 3,003,938 + BPHYT_RS13155 0.13 -0.4 3,003,989 - BPHYT_RS13155 0.22 -0.2 3,004,004 + BPHYT_RS13155 0.24 -1.4 3,004,005 - BPHYT_RS13155 0.25 +0.5 3,004,278 - BPHYT_RS13155 0.72 -0.2 3,004,278 - BPHYT_RS13155 0.72 -0.8 3,004,538 + -0.2
Or see this region's nucleotide sequence