Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13130 and BPHYT_RS13135 are separated by 10 nucleotides BPHYT_RS13135 and BPHYT_RS13140 are separated by 209 nucleotides BPHYT_RS13140 and BPHYT_RS13145 are separated by 28 nucleotides BPHYT_RS13145 and BPHYT_RS13150 are separated by 20 nucleotides
BPHYT_RS13130: BPHYT_RS13130 - metallophosphatase, at 2,999,730 to 3,000,557
_RS13130
BPHYT_RS13135: BPHYT_RS13135 - hypothetical protein, at 3,000,568 to 3,001,053
_RS13135
BPHYT_RS13140: BPHYT_RS13140 - MFS transporter, at 3,001,263 to 3,002,462
_RS13140
BPHYT_RS13145: BPHYT_RS13145 - chemotaxis protein CheY, at 3,002,491 to 3,002,919
_RS13145
BPHYT_RS13150: BPHYT_RS13150 - hisitidine kinase, at 3,002,940 to 3,003,572
_RS13150
Position (kb)
3001
3002
3003 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3000.495 kb on + strand at 3000.495 kb on + strand at 3000.496 kb on - strand at 3000.717 kb on - strand, within BPHYT_RS13135 at 3000.967 kb on - strand, within BPHYT_RS13135 at 3000.968 kb on + strand, within BPHYT_RS13135 at 3000.969 kb on - strand, within BPHYT_RS13135 at 3001.041 kb on + strand at 3001.068 kb on - strand at 3001.869 kb on + strand, within BPHYT_RS13140 at 3001.967 kb on + strand, within BPHYT_RS13140 at 3001.968 kb on - strand, within BPHYT_RS13140 at 3002.304 kb on + strand, within BPHYT_RS13140 at 3002.305 kb on - strand, within BPHYT_RS13140 at 3002.317 kb on - strand, within BPHYT_RS13140 at 3002.926 kb on - strand at 3002.971 kb on + strand at 3003.025 kb on + strand, within BPHYT_RS13150
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 3,000,495 + +1.0 3,000,495 + -1.2 3,000,496 - -0.5 3,000,717 - BPHYT_RS13135 0.31 +0.1 3,000,967 - BPHYT_RS13135 0.82 +0.4 3,000,968 + BPHYT_RS13135 0.82 +1.0 3,000,969 - BPHYT_RS13135 0.83 -2.9 3,001,041 + -0.1 3,001,068 - +0.7 3,001,869 + BPHYT_RS13140 0.51 -0.4 3,001,967 + BPHYT_RS13140 0.59 -1.5 3,001,968 - BPHYT_RS13140 0.59 +0.7 3,002,304 + BPHYT_RS13140 0.87 -0.5 3,002,305 - BPHYT_RS13140 0.87 +0.7 3,002,317 - BPHYT_RS13140 0.88 -0.8 3,002,926 - -0.7 3,002,971 + +1.4 3,003,025 + BPHYT_RS13150 0.13 +0.8
Or see this region's nucleotide sequence