Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12610 and BPHYT_RS12615 are separated by 132 nucleotides BPHYT_RS12615 and BPHYT_RS12620 are separated by 43 nucleotides BPHYT_RS12620 and BPHYT_RS12625 are separated by 35 nucleotides
BPHYT_RS12610: BPHYT_RS12610 - lipoprotein, at 2,877,991 to 2,879,130
_RS12610
BPHYT_RS12615: BPHYT_RS12615 - dihydrodipicolinate synthase, at 2,879,263 to 2,880,189
_RS12615
BPHYT_RS12620: BPHYT_RS12620 - SAM-dependent methyltransferase PhcB, at 2,880,233 to 2,880,835
_RS12620
BPHYT_RS12625: BPHYT_RS12625 - tryptophanyl-tRNA synthetase, at 2,880,871 to 2,882,073
_RS12625
Position (kb)
2879
2880
2881 Strain fitness (log2 ratio)
-2
-1
0
1 at 2879.745 kb on - strand, within BPHYT_RS12615 at 2880.249 kb on + strand at 2880.431 kb on - strand, within BPHYT_RS12620 at 2880.830 kb on - strand at 2880.830 kb on - strand at 2880.830 kb on - strand at 2880.830 kb on - strand at 2880.901 kb on + strand at 2880.902 kb on - strand at 2880.990 kb on - strand at 2880.990 kb on - strand at 2880.990 kb on - strand at 2881.011 kb on + strand, within BPHYT_RS12625 at 2881.011 kb on + strand, within BPHYT_RS12625 at 2881.012 kb on - strand, within BPHYT_RS12625 at 2881.012 kb on - strand, within BPHYT_RS12625 at 2881.012 kb on - strand, within BPHYT_RS12625
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 2,879,745 - BPHYT_RS12615 0.52 +0.3 2,880,249 + -1.4 2,880,431 - BPHYT_RS12620 0.33 -0.1 2,880,830 - +0.5 2,880,830 - +0.9 2,880,830 - +0.6 2,880,830 - -1.2 2,880,901 + -0.2 2,880,902 - +1.5 2,880,990 - -0.2 2,880,990 - +0.6 2,880,990 - +1.6 2,881,011 + BPHYT_RS12625 0.12 +0.5 2,881,011 + BPHYT_RS12625 0.12 +1.6 2,881,012 - BPHYT_RS12625 0.12 +1.2 2,881,012 - BPHYT_RS12625 0.12 -2.4 2,881,012 - BPHYT_RS12625 0.12 -0.6
Or see this region's nucleotide sequence