Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11150

Experiment: 36hr PHYTO1 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11145 and BPHYT_RS11150 are separated by 251 nucleotidesBPHYT_RS11150 and BPHYT_RS11155 are separated by 53 nucleotidesBPHYT_RS11155 and BPHYT_RS11160 are separated by 174 nucleotidesBPHYT_RS11160 and BPHYT_RS11165 are separated by 212 nucleotides BPHYT_RS11145: BPHYT_RS11145 - short-chain dehydrogenase, at 2,533,046 to 2,533,819 _RS11145 BPHYT_RS11150: BPHYT_RS11150 - LysR family transcriptional regulator, at 2,534,071 to 2,535,000 _RS11150 BPHYT_RS11155: BPHYT_RS11155 - hypothetical protein, at 2,535,054 to 2,535,305 _RS11155 BPHYT_RS11160: BPHYT_RS11160 - hypothetical protein, at 2,535,480 to 2,535,659 _RS11160 BPHYT_RS11165: BPHYT_RS11165 - glycosyl transferase, at 2,535,872 to 2,536,954 _RS11165 Position (kb) 2534 2535 2536Strain fitness (log2 ratio) -2 -1 0 1 2at 2533.847 kb on - strandat 2533.979 kb on - strandat 2533.982 kb on - strandat 2534.065 kb on + strandat 2534.065 kb on + strandat 2534.555 kb on + strand, within BPHYT_RS11150at 2534.897 kb on - strand, within BPHYT_RS11150at 2535.092 kb on - strand, within BPHYT_RS11155at 2535.311 kb on - strandat 2535.663 kb on - strandat 2535.855 kb on + strandat 2535.871 kb on + strandat 2535.871 kb on + strandat 2535.871 kb on + strandat 2535.872 kb on - strandat 2535.983 kb on + strand, within BPHYT_RS11165

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1
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2,533,847 - -1.8
2,533,979 - -1.0
2,533,982 - -0.6
2,534,065 + -0.2
2,534,065 + +0.2
2,534,555 + BPHYT_RS11150 0.52 -0.7
2,534,897 - BPHYT_RS11150 0.89 -0.7
2,535,092 - BPHYT_RS11155 0.15 -0.0
2,535,311 - +2.7
2,535,663 - +1.0
2,535,855 + +1.3
2,535,871 + -0.7
2,535,871 + -0.9
2,535,871 + +0.2
2,535,872 - +0.7
2,535,983 + BPHYT_RS11165 0.10 +0.1

Or see this region's nucleotide sequence