Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10875 and BPHYT_RS10880 are separated by 169 nucleotides BPHYT_RS10880 and BPHYT_RS10885 overlap by 11 nucleotides BPHYT_RS10885 and BPHYT_RS10890 are separated by 12 nucleotides BPHYT_RS10890 and BPHYT_RS10895 are separated by 179 nucleotides
BPHYT_RS10875: BPHYT_RS10875 - transporter, at 2,473,874 to 2,476,924
_RS10875
BPHYT_RS10880: BPHYT_RS10880 - hopanoid biosynthesis associated protein HpnK, at 2,477,094 to 2,477,918
_RS10880
BPHYT_RS10885: BPHYT_RS10885 - glycosyl transferase, at 2,477,908 to 2,479,215
_RS10885
BPHYT_RS10890: BPHYT_RS10890 - hypothetical protein, at 2,479,228 to 2,479,578
_RS10890
BPHYT_RS10895: BPHYT_RS10895 - transcriptional regulator, at 2,479,758 to 2,480,417
_RS10895
Position (kb)
2477
2478
2479
2480 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2477.095 kb on + strand at 2478.079 kb on - strand, within BPHYT_RS10885 at 2478.331 kb on - strand, within BPHYT_RS10885 at 2478.971 kb on + strand, within BPHYT_RS10885 at 2479.080 kb on - strand, within BPHYT_RS10885 at 2479.645 kb on - strand at 2479.709 kb on - strand at 2479.757 kb on + strand at 2479.758 kb on - strand at 2479.758 kb on - strand at 2479.765 kb on + strand at 2479.765 kb on + strand at 2480.050 kb on - strand, within BPHYT_RS10895
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 2,477,095 + +0.1 2,478,079 - BPHYT_RS10885 0.13 +0.1 2,478,331 - BPHYT_RS10885 0.32 -0.4 2,478,971 + BPHYT_RS10885 0.81 -1.6 2,479,080 - BPHYT_RS10885 0.90 -0.9 2,479,645 - +0.2 2,479,709 - -1.1 2,479,757 + +0.0 2,479,758 - -0.9 2,479,758 - +2.5 2,479,765 + -1.8 2,479,765 + +0.2 2,480,050 - BPHYT_RS10895 0.44 -1.1
Or see this region's nucleotide sequence