Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09375 and BPHYT_RS09380 overlap by 11 nucleotides BPHYT_RS09380 and BPHYT_RS09385 are separated by 7 nucleotides BPHYT_RS09385 and BPHYT_RS09390 overlap by 4 nucleotides BPHYT_RS09390 and BPHYT_RS09395 are separated by 16 nucleotides
BPHYT_RS09375: BPHYT_RS09375 - (2Fe-2S)-binding protein, at 2,123,854 to 2,125,260
_RS09375
BPHYT_RS09380: BPHYT_RS09380 - NAD(FAD)-dependent dehydrogenase, at 2,125,250 to 2,125,630
_RS09380
BPHYT_RS09385: BPHYT_RS09385 - FAD-dependent oxidoreductase, at 2,125,638 to 2,126,774
_RS09385
BPHYT_RS09390: BPHYT_RS09390 - ABC transporter permease, at 2,126,771 to 2,127,568
_RS09390
BPHYT_RS09395: BPHYT_RS09395 - ABC transporter permease, at 2,127,585 to 2,128,457
_RS09395
Position (kb)
2125
2126
2127 Strain fitness (log2 ratio)
-2
-1
0
1 at 2124.667 kb on - strand, within BPHYT_RS09375 at 2124.667 kb on - strand, within BPHYT_RS09375 at 2124.743 kb on + strand, within BPHYT_RS09375 at 2124.761 kb on + strand, within BPHYT_RS09375 at 2124.959 kb on + strand, within BPHYT_RS09375 at 2124.959 kb on + strand, within BPHYT_RS09375 at 2124.960 kb on - strand, within BPHYT_RS09375 at 2125.115 kb on + strand, within BPHYT_RS09375 at 2126.594 kb on - strand, within BPHYT_RS09385 at 2126.667 kb on + strand at 2126.908 kb on - strand, within BPHYT_RS09390 at 2126.908 kb on - strand, within BPHYT_RS09390 at 2126.908 kb on - strand, within BPHYT_RS09390 at 2127.523 kb on + strand at 2127.523 kb on + strand at 2127.537 kb on + strand at 2127.718 kb on + strand, within BPHYT_RS09395
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 2,124,667 - BPHYT_RS09375 0.58 -0.5 2,124,667 - BPHYT_RS09375 0.58 +0.0 2,124,743 + BPHYT_RS09375 0.63 +0.7 2,124,761 + BPHYT_RS09375 0.64 -0.4 2,124,959 + BPHYT_RS09375 0.79 +0.5 2,124,959 + BPHYT_RS09375 0.79 -1.7 2,124,960 - BPHYT_RS09375 0.79 -0.8 2,125,115 + BPHYT_RS09375 0.90 -2.6 2,126,594 - BPHYT_RS09385 0.84 -1.5 2,126,667 + +1.4 2,126,908 - BPHYT_RS09390 0.17 -0.4 2,126,908 - BPHYT_RS09390 0.17 +0.4 2,126,908 - BPHYT_RS09390 0.17 +0.6 2,127,523 + -0.8 2,127,523 + -1.1 2,127,537 + +0.2 2,127,718 + BPHYT_RS09395 0.15 -0.6
Or see this region's nucleotide sequence