Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09355 and BPHYT_RS09360 are separated by 195 nucleotides BPHYT_RS09360 and BPHYT_RS09365 are separated by 85 nucleotides BPHYT_RS09365 and BPHYT_RS09370 are separated by 73 nucleotides
BPHYT_RS09355: BPHYT_RS09355 - LysR family transcriptional regulator, at 2,120,368 to 2,121,276
_RS09355
BPHYT_RS09360: BPHYT_RS09360 - short-chain dehydrogenase/reductase SDR, at 2,121,472 to 2,122,176
_RS09360
BPHYT_RS09365: BPHYT_RS09365 - cytochrome C2, at 2,122,262 to 2,122,615
_RS09365
BPHYT_RS09370: BPHYT_RS09370 - FAD-dependent oxidoreductase, at 2,122,689 to 2,123,861
_RS09370
Position (kb)
2122
2123 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2121.291 kb on - strand at 2121.305 kb on - strand at 2121.320 kb on + strand at 2121.321 kb on - strand at 2121.322 kb on + strand at 2121.323 kb on - strand at 2121.324 kb on - strand at 2121.325 kb on + strand at 2121.647 kb on + strand, within BPHYT_RS09360 at 2121.647 kb on + strand, within BPHYT_RS09360 at 2121.648 kb on - strand, within BPHYT_RS09360 at 2121.716 kb on + strand, within BPHYT_RS09360 at 2121.717 kb on - strand, within BPHYT_RS09360 at 2121.767 kb on + strand, within BPHYT_RS09360 at 2121.903 kb on + strand, within BPHYT_RS09360 at 2121.904 kb on - strand, within BPHYT_RS09360 at 2121.929 kb on - strand, within BPHYT_RS09360 at 2122.104 kb on + strand, within BPHYT_RS09360 at 2122.142 kb on - strand at 2122.547 kb on + strand, within BPHYT_RS09365 at 2122.547 kb on + strand, within BPHYT_RS09365 at 2122.920 kb on - strand, within BPHYT_RS09370 at 2123.507 kb on + strand, within BPHYT_RS09370 at 2123.511 kb on + strand, within BPHYT_RS09370
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 2,121,291 - +0.4 2,121,305 - +0.4 2,121,320 + +0.5 2,121,321 - +1.2 2,121,322 + -0.3 2,121,323 - +1.3 2,121,324 - +0.5 2,121,325 + +2.0 2,121,647 + BPHYT_RS09360 0.25 +0.6 2,121,647 + BPHYT_RS09360 0.25 +0.2 2,121,648 - BPHYT_RS09360 0.25 +0.3 2,121,716 + BPHYT_RS09360 0.35 +0.5 2,121,717 - BPHYT_RS09360 0.35 -0.9 2,121,767 + BPHYT_RS09360 0.42 +3.5 2,121,903 + BPHYT_RS09360 0.61 +0.5 2,121,904 - BPHYT_RS09360 0.61 +0.2 2,121,929 - BPHYT_RS09360 0.65 -0.6 2,122,104 + BPHYT_RS09360 0.90 -1.5 2,122,142 - -0.9 2,122,547 + BPHYT_RS09365 0.81 -1.0 2,122,547 + BPHYT_RS09365 0.81 -0.7 2,122,920 - BPHYT_RS09370 0.20 -0.1 2,123,507 + BPHYT_RS09370 0.70 -0.8 2,123,511 + BPHYT_RS09370 0.70 +0.1
Or see this region's nucleotide sequence