Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08635 and BPHYT_RS08640 are separated by 504 nucleotides BPHYT_RS08640 and BPHYT_RS08645 are separated by 51 nucleotides BPHYT_RS08645 and BPHYT_RS08650 overlap by 4 nucleotides
BPHYT_RS08635: BPHYT_RS08635 - membrane protein, at 1,968,293 to 1,968,718
_RS08635
BPHYT_RS08640: BPHYT_RS08640 - alkanesulfonate monooxygenase, at 1,969,223 to 1,970,383
_RS08640
BPHYT_RS08645: BPHYT_RS08645 - sulfonate ABC transporter, at 1,970,435 to 1,971,277
_RS08645
BPHYT_RS08650: BPHYT_RS08650 - sulfonate ABC transporter ATP-binding protein, at 1,971,274 to 1,972,275
_RS08650
Position (kb)
1969
1970
1971 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1968.771 kb on + strand at 1968.792 kb on + strand at 1968.793 kb on - strand at 1968.793 kb on - strand at 1968.793 kb on - strand at 1968.793 kb on - strand at 1968.793 kb on - strand at 1968.794 kb on + strand at 1968.794 kb on + strand at 1968.795 kb on - strand at 1968.795 kb on - strand at 1968.795 kb on - strand at 1968.796 kb on + strand at 1968.796 kb on + strand at 1968.796 kb on + strand at 1968.796 kb on + strand at 1968.797 kb on - strand at 1968.797 kb on - strand at 1968.797 kb on - strand at 1968.798 kb on + strand at 1968.799 kb on - strand at 1968.805 kb on - strand at 1968.805 kb on - strand at 1968.847 kb on + strand at 1968.879 kb on + strand at 1969.192 kb on + strand at 1969.347 kb on - strand, within BPHYT_RS08640 at 1969.719 kb on - strand, within BPHYT_RS08640 at 1969.798 kb on + strand, within BPHYT_RS08640 at 1969.798 kb on + strand, within BPHYT_RS08640 at 1970.253 kb on - strand, within BPHYT_RS08640 at 1970.354 kb on + strand at 1970.355 kb on - strand at 1970.355 kb on - strand at 1970.906 kb on + strand, within BPHYT_RS08645 at 1971.255 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 1,968,771 + +1.5 1,968,792 + -0.1 1,968,793 - +0.2 1,968,793 - +0.8 1,968,793 - -0.2 1,968,793 - -0.8 1,968,793 - -0.4 1,968,794 + +0.9 1,968,794 + -0.1 1,968,795 - -1.0 1,968,795 - +0.4 1,968,795 - +0.0 1,968,796 + -2.8 1,968,796 + -0.5 1,968,796 + +0.9 1,968,796 + +0.7 1,968,797 - -0.8 1,968,797 - -0.5 1,968,797 - -0.1 1,968,798 + +0.4 1,968,799 - -0.7 1,968,805 - +0.7 1,968,805 - -1.3 1,968,847 + -0.1 1,968,879 + -1.8 1,969,192 + +0.4 1,969,347 - BPHYT_RS08640 0.11 -1.5 1,969,719 - BPHYT_RS08640 0.43 -0.6 1,969,798 + BPHYT_RS08640 0.50 -1.8 1,969,798 + BPHYT_RS08640 0.50 -0.2 1,970,253 - BPHYT_RS08640 0.89 +0.2 1,970,354 + -0.7 1,970,355 - -0.3 1,970,355 - +0.4 1,970,906 + BPHYT_RS08645 0.56 -1.2 1,971,255 - -0.2
Or see this region's nucleotide sequence