Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04875 and BPHYT_RS04880 are separated by 29 nucleotides BPHYT_RS04880 and BPHYT_RS04885 are separated by 62 nucleotides BPHYT_RS04885 and BPHYT_RS04890 are separated by 17 nucleotides BPHYT_RS04890 and BPHYT_RS04895 overlap by 1 nucleotides
BPHYT_RS04875: BPHYT_RS04875 - alpha-ribazole phosphatase, at 1,122,320 to 1,122,910
_RS04875
BPHYT_RS04880: BPHYT_RS04880 - cobalamin-binding protein, at 1,122,940 to 1,123,842
_RS04880
BPHYT_RS04885: BPHYT_RS04885 - threonine-phosphate decarboxylase, at 1,123,905 to 1,124,954
_RS04885
BPHYT_RS04890: BPHYT_RS04890 - cobalamin biosynthesis protein CobD, at 1,124,972 to 1,125,907
_RS04890
BPHYT_RS04895: BPHYT_RS04895 - adenosylcobinamide kinase, at 1,125,907 to 1,126,461
_RS04895
Position (kb)
1123
1124
1125 Strain fitness (log2 ratio)
-2
-1
0
1 at 1122.922 kb on + strand at 1122.922 kb on + strand at 1122.922 kb on + strand at 1122.923 kb on - strand at 1122.923 kb on - strand at 1122.928 kb on - strand at 1122.928 kb on - strand at 1123.316 kb on + strand, within BPHYT_RS04880 at 1123.379 kb on + strand, within BPHYT_RS04880 at 1123.688 kb on + strand, within BPHYT_RS04880 at 1123.688 kb on + strand, within BPHYT_RS04880 at 1123.688 kb on + strand, within BPHYT_RS04880 at 1123.689 kb on - strand, within BPHYT_RS04880 at 1123.865 kb on - strand at 1123.865 kb on - strand at 1124.620 kb on + strand, within BPHYT_RS04885 at 1124.624 kb on + strand, within BPHYT_RS04885 at 1124.831 kb on + strand, within BPHYT_RS04885 at 1125.038 kb on - strand at 1125.268 kb on - strand, within BPHYT_RS04890 at 1125.268 kb on - strand, within BPHYT_RS04890
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 1,122,922 + +0.4 1,122,922 + +0.7 1,122,922 + -0.4 1,122,923 - -0.3 1,122,923 - +0.4 1,122,928 - +1.7 1,122,928 - +0.2 1,123,316 + BPHYT_RS04880 0.42 -0.8 1,123,379 + BPHYT_RS04880 0.49 -0.2 1,123,688 + BPHYT_RS04880 0.83 +0.1 1,123,688 + BPHYT_RS04880 0.83 -0.1 1,123,688 + BPHYT_RS04880 0.83 -0.7 1,123,689 - BPHYT_RS04880 0.83 -0.7 1,123,865 - -0.3 1,123,865 - -0.2 1,124,620 + BPHYT_RS04885 0.68 +0.2 1,124,624 + BPHYT_RS04885 0.68 -1.2 1,124,831 + BPHYT_RS04885 0.88 +0.5 1,125,038 - +0.8 1,125,268 - BPHYT_RS04890 0.32 -0.0 1,125,268 - BPHYT_RS04890 0.32 -2.2
Or see this region's nucleotide sequence