Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03160 and BPHYT_RS03165 are separated by 67 nucleotides BPHYT_RS03165 and BPHYT_RS03170 are separated by 38 nucleotides BPHYT_RS03170 and BPHYT_RS03175 are separated by 157 nucleotides BPHYT_RS03175 and BPHYT_RS03180 are separated by 15 nucleotides
BPHYT_RS03160: BPHYT_RS03160 - hypothetical protein, at 709,627 to 710,040
_RS03160
BPHYT_RS03165: BPHYT_RS03165 - ubiquinone/menaquinone biosynthesis C-methyltransferase UbiE, at 710,108 to 710,839
_RS03165
BPHYT_RS03170: BPHYT_RS03170 - membrane protein, at 710,878 to 711,870
_RS03170
BPHYT_RS03175: BPHYT_RS03175 - sterol-binding protein, at 712,028 to 712,672
_RS03175
BPHYT_RS03180: BPHYT_RS03180 - hypothetical protein, at 712,688 to 714,265
_RS03180
Position (kb) 710
711
712 Strain fitness (log2 ratio) -1
0
1
2 at 709.900 kb on + strand, within BPHYT_RS03160 at 709.900 kb on + strand, within BPHYT_RS03160 at 709.900 kb on + strand, within BPHYT_RS03160 at 710.041 kb on + strand at 710.041 kb on + strand at 710.088 kb on + strand at 710.835 kb on - strand at 711.042 kb on + strand, within BPHYT_RS03170 at 711.316 kb on - strand, within BPHYT_RS03170 at 711.539 kb on - strand, within BPHYT_RS03170 at 711.945 kb on + strand at 711.971 kb on + strand at 711.971 kb on + strand at 711.971 kb on + strand at 711.971 kb on + strand at 711.972 kb on - strand at 712.263 kb on + strand, within BPHYT_RS03175 at 712.263 kb on + strand, within BPHYT_RS03175 at 712.670 kb on + strand at 712.670 kb on + strand at 712.687 kb on + strand at 712.687 kb on + strand at 712.687 kb on + strand at 712.687 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 709,900 + BPHYT_RS03160 0.66 -1.0 709,900 + BPHYT_RS03160 0.66 -0.7 709,900 + BPHYT_RS03160 0.66 -1.0 710,041 + +0.6 710,041 + -0.2 710,088 + +0.1 710,835 - +1.5 711,042 + BPHYT_RS03170 0.17 -1.1 711,316 - BPHYT_RS03170 0.44 +2.5 711,539 - BPHYT_RS03170 0.67 +1.5 711,945 + +0.2 711,971 + +0.2 711,971 + -1.1 711,971 + -0.1 711,971 + -0.1 711,972 - +0.0 712,263 + BPHYT_RS03175 0.36 +0.2 712,263 + BPHYT_RS03175 0.36 -0.7 712,670 + +2.3 712,670 + -0.3 712,687 + -0.8 712,687 + -0.1 712,687 + -0.3 712,687 + +0.1
Or see this region's nucleotide sequence