Experiment: 36hr PHYTO1 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS01840 and BPHYT_RS01845 are separated by 88 nucleotides BPHYT_RS01845 and BPHYT_RS01850 are separated by 268 nucleotides BPHYT_RS01850 and BPHYT_RS01855 are separated by 418 nucleotides
BPHYT_RS01840: BPHYT_RS01840 - endonuclease, at 418,275 to 419,072
_RS01840
BPHYT_RS01845: BPHYT_RS01845 - hypothetical protein, at 419,161 to 419,700
_RS01845
BPHYT_RS01850: BPHYT_RS01850 - ATPase AAA, at 419,969 to 420,946
_RS01850
BPHYT_RS01855: BPHYT_RS01855 - FAD-dependent oxidoreductase, at 421,365 to 422,471
_RS01855
Position (kb) 419
420
421 Strain fitness (log2 ratio) -2
-1
0
1
2 at 418.997 kb on + strand at 418.998 kb on - strand at 419.038 kb on - strand at 419.472 kb on + strand, within BPHYT_RS01845 at 419.700 kb on + strand at 419.701 kb on - strand at 419.751 kb on + strand at 419.752 kb on - strand at 419.752 kb on - strand at 419.761 kb on - strand at 419.761 kb on - strand at 419.821 kb on + strand at 419.891 kb on + strand at 419.891 kb on + strand at 419.926 kb on + strand at 419.927 kb on - strand at 419.927 kb on - strand at 419.927 kb on - strand at 419.927 kb on - strand at 420.024 kb on - strand at 420.121 kb on + strand, within BPHYT_RS01850 at 420.341 kb on + strand, within BPHYT_RS01850 at 420.347 kb on + strand, within BPHYT_RS01850 at 420.347 kb on + strand, within BPHYT_RS01850 at 420.347 kb on + strand, within BPHYT_RS01850 at 420.348 kb on - strand, within BPHYT_RS01850 at 420.455 kb on + strand, within BPHYT_RS01850 at 420.456 kb on - strand, within BPHYT_RS01850 at 420.512 kb on + strand, within BPHYT_RS01850 at 420.512 kb on + strand, within BPHYT_RS01850 at 420.512 kb on + strand, within BPHYT_RS01850 at 420.512 kb on + strand, within BPHYT_RS01850 at 420.513 kb on - strand, within BPHYT_RS01850 at 420.513 kb on - strand, within BPHYT_RS01850 at 420.513 kb on - strand, within BPHYT_RS01850 at 420.533 kb on + strand, within BPHYT_RS01850 at 420.533 kb on + strand, within BPHYT_RS01850 at 420.533 kb on + strand, within BPHYT_RS01850 at 420.533 kb on + strand, within BPHYT_RS01850 at 420.533 kb on + strand, within BPHYT_RS01850 at 420.534 kb on - strand, within BPHYT_RS01850 at 420.534 kb on - strand, within BPHYT_RS01850 at 420.534 kb on - strand, within BPHYT_RS01850 at 420.677 kb on + strand, within BPHYT_RS01850 at 420.677 kb on + strand, within BPHYT_RS01850 at 420.677 kb on + strand, within BPHYT_RS01850 at 420.677 kb on + strand, within BPHYT_RS01850 at 420.678 kb on - strand, within BPHYT_RS01850 at 420.678 kb on - strand, within BPHYT_RS01850 at 420.678 kb on - strand, within BPHYT_RS01850 at 420.731 kb on + strand, within BPHYT_RS01850 at 420.754 kb on + strand, within BPHYT_RS01850 at 420.755 kb on - strand, within BPHYT_RS01850 at 420.995 kb on - strand at 421.047 kb on - strand at 421.096 kb on + strand at 421.096 kb on + strand at 421.097 kb on - strand at 421.098 kb on - strand at 421.131 kb on - strand at 421.131 kb on - strand at 421.156 kb on + strand at 421.157 kb on - strand at 421.157 kb on - strand at 421.157 kb on - strand at 421.217 kb on + strand at 421.281 kb on + strand at 421.282 kb on - strand at 421.384 kb on + strand at 421.384 kb on + strand at 421.527 kb on + strand, within BPHYT_RS01855 at 421.527 kb on + strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.528 kb on - strand, within BPHYT_RS01855 at 421.584 kb on + strand, within BPHYT_RS01855 at 421.585 kb on - strand, within BPHYT_RS01855 at 421.585 kb on - strand, within BPHYT_RS01855 at 421.585 kb on - strand, within BPHYT_RS01855 at 421.590 kb on + strand, within BPHYT_RS01855 at 421.591 kb on - strand, within BPHYT_RS01855
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 1 remove 418,997 + +0.3 418,998 - +0.6 419,038 - -1.2 419,472 + BPHYT_RS01845 0.58 +0.6 419,700 + +0.5 419,701 - +0.9 419,751 + -0.3 419,752 - -0.1 419,752 - -2.6 419,761 - -0.2 419,761 - -0.2 419,821 + +0.0 419,891 + -0.1 419,891 + +0.1 419,926 + -0.4 419,927 - -1.1 419,927 - -1.7 419,927 - +0.1 419,927 - +1.3 420,024 - -0.4 420,121 + BPHYT_RS01850 0.16 -0.1 420,341 + BPHYT_RS01850 0.38 +0.6 420,347 + BPHYT_RS01850 0.39 -0.1 420,347 + BPHYT_RS01850 0.39 +0.1 420,347 + BPHYT_RS01850 0.39 -0.8 420,348 - BPHYT_RS01850 0.39 +0.0 420,455 + BPHYT_RS01850 0.50 -0.0 420,456 - BPHYT_RS01850 0.50 -0.9 420,512 + BPHYT_RS01850 0.56 +0.3 420,512 + BPHYT_RS01850 0.56 +0.2 420,512 + BPHYT_RS01850 0.56 -1.0 420,512 + BPHYT_RS01850 0.56 -1.7 420,513 - BPHYT_RS01850 0.56 +0.0 420,513 - BPHYT_RS01850 0.56 -0.8 420,513 - BPHYT_RS01850 0.56 +1.4 420,533 + BPHYT_RS01850 0.58 +0.3 420,533 + BPHYT_RS01850 0.58 -1.5 420,533 + BPHYT_RS01850 0.58 +1.0 420,533 + BPHYT_RS01850 0.58 -0.6 420,533 + BPHYT_RS01850 0.58 -0.0 420,534 - BPHYT_RS01850 0.58 -1.0 420,534 - BPHYT_RS01850 0.58 -1.7 420,534 - BPHYT_RS01850 0.58 -1.4 420,677 + BPHYT_RS01850 0.72 -0.7 420,677 + BPHYT_RS01850 0.72 +0.2 420,677 + BPHYT_RS01850 0.72 +1.2 420,677 + BPHYT_RS01850 0.72 -0.6 420,678 - BPHYT_RS01850 0.72 -1.9 420,678 - BPHYT_RS01850 0.72 -0.3 420,678 - BPHYT_RS01850 0.72 +0.4 420,731 + BPHYT_RS01850 0.78 -0.4 420,754 + BPHYT_RS01850 0.80 -1.2 420,755 - BPHYT_RS01850 0.80 -1.1 420,995 - -0.3 421,047 - +0.2 421,096 + -1.4 421,096 + -0.2 421,097 - -0.1 421,098 - +1.4 421,131 - -1.5 421,131 - +0.6 421,156 + -0.2 421,157 - -1.0 421,157 - +0.5 421,157 - -0.3 421,217 + +1.9 421,281 + -0.9 421,282 - -1.6 421,384 + +0.2 421,384 + +0.3 421,527 + BPHYT_RS01855 0.15 +1.4 421,527 + BPHYT_RS01855 0.15 -0.6 421,528 - BPHYT_RS01855 0.15 -0.1 421,528 - BPHYT_RS01855 0.15 -0.5 421,528 - BPHYT_RS01855 0.15 +1.4 421,528 - BPHYT_RS01855 0.15 +0.5 421,528 - BPHYT_RS01855 0.15 -0.5 421,528 - BPHYT_RS01855 0.15 -0.2 421,528 - BPHYT_RS01855 0.15 -0.5 421,584 + BPHYT_RS01855 0.20 -1.1 421,585 - BPHYT_RS01855 0.20 -0.7 421,585 - BPHYT_RS01855 0.20 -0.6 421,585 - BPHYT_RS01855 0.20 -0.4 421,590 + BPHYT_RS01855 0.20 -0.1 421,591 - BPHYT_RS01855 0.20 +2.2
Or see this region's nucleotide sequence