Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS35705

Experiment: 36hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35700 and BPHYT_RS35705 are separated by 53 nucleotidesBPHYT_RS35705 and BPHYT_RS35710 are separated by 65 nucleotidesBPHYT_RS35710 and BPHYT_RS35715 are separated by 310 nucleotides BPHYT_RS35700: BPHYT_RS35700 - beta-lactamase domain-containing protein, at 3,600,852 to 3,601,811 _RS35700 BPHYT_RS35705: BPHYT_RS35705 - FAD-dependent oxidoreductase, at 3,601,865 to 3,603,574 _RS35705 BPHYT_RS35710: BPHYT_RS35710 - hypothetical protein, at 3,603,640 to 3,603,876 _RS35710 BPHYT_RS35715: BPHYT_RS35715 - hypothetical protein, at 3,604,187 to 3,604,663 _RS35715 Position (kb) 3601 3602 3603 3604Strain fitness (log2 ratio) -1 0 1at 3600.928 kb on - strandat 3601.072 kb on - strand, within BPHYT_RS35700at 3601.123 kb on - strand, within BPHYT_RS35700at 3601.167 kb on + strand, within BPHYT_RS35700at 3601.167 kb on + strand, within BPHYT_RS35700at 3601.167 kb on + strand, within BPHYT_RS35700at 3601.168 kb on - strand, within BPHYT_RS35700at 3601.168 kb on - strand, within BPHYT_RS35700at 3601.877 kb on + strandat 3602.189 kb on + strand, within BPHYT_RS35705at 3602.424 kb on - strand, within BPHYT_RS35705at 3603.977 kb on + strandat 3604.027 kb on - strandat 3604.053 kb on + strandat 3604.205 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10
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3,600,928 - -0.4
3,601,072 - BPHYT_RS35700 0.23 -0.6
3,601,123 - BPHYT_RS35700 0.28 +1.0
3,601,167 + BPHYT_RS35700 0.33 +0.5
3,601,167 + BPHYT_RS35700 0.33 -0.0
3,601,167 + BPHYT_RS35700 0.33 -1.2
3,601,168 - BPHYT_RS35700 0.33 +1.6
3,601,168 - BPHYT_RS35700 0.33 -0.4
3,601,877 + -1.0
3,602,189 + BPHYT_RS35705 0.19 -0.8
3,602,424 - BPHYT_RS35705 0.33 -0.9
3,603,977 + +0.1
3,604,027 - -0.3
3,604,053 + +0.8
3,604,205 - -0.4

Or see this region's nucleotide sequence