Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32525

Experiment: 36hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32520 and BPHYT_RS32525 are separated by 667 nucleotidesBPHYT_RS32525 and BPHYT_RS32530 are separated by 250 nucleotides BPHYT_RS32520: BPHYT_RS32520 - GntR family transcriptional regulator, at 2,867,705 to 2,868,553 _RS32520 BPHYT_RS32525: BPHYT_RS32525 - hypothetical protein, at 2,869,221 to 2,869,685 _RS32525 BPHYT_RS32530: BPHYT_RS32530 - transposase, at 2,869,936 to 2,871,492 _RS32530 Position (kb) 2869 2870Strain fitness (log2 ratio) -2 -1 0 1 2at 2868.247 kb on + strand, within BPHYT_RS32520at 2868.437 kb on + strand, within BPHYT_RS32520at 2868.438 kb on - strand, within BPHYT_RS32520at 2868.646 kb on - strandat 2868.651 kb on + strandat 2868.652 kb on - strandat 2869.140 kb on + strandat 2869.186 kb on + strandat 2869.206 kb on - strandat 2869.232 kb on + strandat 2869.233 kb on - strandat 2869.233 kb on - strandat 2869.340 kb on - strand, within BPHYT_RS32525at 2869.408 kb on + strand, within BPHYT_RS32525at 2869.641 kb on - strandat 2869.670 kb on - strandat 2869.670 kb on - strandat 2869.673 kb on + strandat 2869.866 kb on - strandat 2869.889 kb on + strandat 2869.900 kb on + strandat 2869.979 kb on + strandat 2870.030 kb on + strandat 2870.241 kb on - strand, within BPHYT_RS32530at 2870.558 kb on + strand, within BPHYT_RS32530at 2870.575 kb on + strand, within BPHYT_RS32530at 2870.640 kb on - strand, within BPHYT_RS32530

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10
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2,868,247 + BPHYT_RS32520 0.64 -0.2
2,868,437 + BPHYT_RS32520 0.86 +0.2
2,868,438 - BPHYT_RS32520 0.86 -1.0
2,868,646 - +0.1
2,868,651 + -0.1
2,868,652 - -0.2
2,869,140 + +1.2
2,869,186 + +0.2
2,869,206 - +0.1
2,869,232 + +0.5
2,869,233 - -0.6
2,869,233 - -0.2
2,869,340 - BPHYT_RS32525 0.26 -0.8
2,869,408 + BPHYT_RS32525 0.40 -1.5
2,869,641 - +0.0
2,869,670 - +0.0
2,869,670 - +0.4
2,869,673 + +0.1
2,869,866 - +0.1
2,869,889 + +1.4
2,869,900 + -0.0
2,869,979 + +1.4
2,870,030 + -0.2
2,870,241 - BPHYT_RS32530 0.20 +0.9
2,870,558 + BPHYT_RS32530 0.40 +1.8
2,870,575 + BPHYT_RS32530 0.41 +0.5
2,870,640 - BPHYT_RS32530 0.45 -1.8

Or see this region's nucleotide sequence