Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31030

Experiment: 36hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31020 and BPHYT_RS31025 overlap by 4 nucleotidesBPHYT_RS31025 and BPHYT_RS31030 are separated by 114 nucleotidesBPHYT_RS31030 and BPHYT_RS31035 are separated by 57 nucleotidesBPHYT_RS31035 and BPHYT_RS31040 are separated by 2 nucleotides BPHYT_RS31020: BPHYT_RS31020 - 3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, at 2,514,001 to 2,514,555 _RS31020 BPHYT_RS31025: BPHYT_RS31025 - 2-dehydro-3-deoxyphosphooctonate aldolase, at 2,514,552 to 2,515,412 _RS31025 BPHYT_RS31030: BPHYT_RS31030 - tyrosine protein kinase, at 2,515,527 to 2,517,752 _RS31030 BPHYT_RS31035: BPHYT_RS31035 - protein-tyrosine-phosphatase, at 2,517,810 to 2,518,247 _RS31035 BPHYT_RS31040: BPHYT_RS31040 - sugar transporter, at 2,518,250 to 2,519,407 _RS31040 Position (kb) 2515 2516 2517 2518Strain fitness (log2 ratio) -1 0 1at 2515.298 kb on - strand, within BPHYT_RS31025at 2515.298 kb on - strand, within BPHYT_RS31025at 2515.571 kb on - strandat 2516.262 kb on + strand, within BPHYT_RS31030at 2516.263 kb on - strand, within BPHYT_RS31030at 2516.556 kb on - strand, within BPHYT_RS31030at 2516.945 kb on - strand, within BPHYT_RS31030at 2517.729 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10
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2,515,298 - BPHYT_RS31025 0.87 -1.2
2,515,298 - BPHYT_RS31025 0.87 -0.9
2,515,571 - -0.9
2,516,262 + BPHYT_RS31030 0.33 +0.6
2,516,263 - BPHYT_RS31030 0.33 -0.5
2,516,556 - BPHYT_RS31030 0.46 +0.2
2,516,945 - BPHYT_RS31030 0.64 -0.4
2,517,729 - -1.2

Or see this region's nucleotide sequence