Experiment: 36hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29175 and BPHYT_RS29180 are separated by 13 nucleotides BPHYT_RS29180 and BPHYT_RS29185 overlap by 1 nucleotides BPHYT_RS29185 and BPHYT_RS29190 are separated by 9 nucleotides
BPHYT_RS29175: BPHYT_RS29175 - ABC transporter ATP-binding protein, at 2,101,194 to 2,102,366
_RS29175
BPHYT_RS29180: BPHYT_RS29180 - sugar ABC transporter permease, at 2,102,380 to 2,103,300
_RS29180
BPHYT_RS29185: BPHYT_RS29185 - sugar ABC transporter permease, at 2,103,300 to 2,104,244
_RS29185
BPHYT_RS29190: BPHYT_RS29190 - sugar ABC transporter substrate-binding protein, at 2,104,254 to 2,105,492
_RS29190
Position (kb) 2102
2103
2104 Strain fitness (log2 ratio) -2
-1
0
1 at 2101.702 kb on + strand, within BPHYT_RS29175 at 2101.703 kb on - strand, within BPHYT_RS29175 at 2102.008 kb on + strand, within BPHYT_RS29175 at 2102.361 kb on - strand at 2102.439 kb on - strand at 2102.491 kb on - strand, within BPHYT_RS29180 at 2102.492 kb on + strand, within BPHYT_RS29180 at 2102.732 kb on + strand, within BPHYT_RS29180 at 2103.155 kb on + strand, within BPHYT_RS29180 at 2103.965 kb on - strand, within BPHYT_RS29185
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10 remove 2,101,702 + BPHYT_RS29175 0.43 +1.1 2,101,703 - BPHYT_RS29175 0.43 -1.6 2,102,008 + BPHYT_RS29175 0.69 +0.8 2,102,361 - +0.1 2,102,439 - -0.2 2,102,491 - BPHYT_RS29180 0.12 -0.3 2,102,492 + BPHYT_RS29180 0.12 -1.3 2,102,732 + BPHYT_RS29180 0.38 -2.1 2,103,155 + BPHYT_RS29180 0.84 -0.5 2,103,965 - BPHYT_RS29185 0.70 -0.4
Or see this region's nucleotide sequence