Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS21425

Experiment: 36hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS21415 and BPHYT_RS21420 are separated by 13 nucleotidesBPHYT_RS21420 and BPHYT_RS21425 are separated by 92 nucleotidesBPHYT_RS21425 and BPHYT_RS21430 are separated by 5 nucleotidesBPHYT_RS21430 and BPHYT_RS21435 overlap by 4 nucleotides BPHYT_RS21415: BPHYT_RS21415 - 3-oxoadipate CoA-transferase subunit A, at 363,604 to 364,311 _RS21415 BPHYT_RS21420: BPHYT_RS21420 - 3-oxoadipate CoA-transferase subunit B, at 364,325 to 364,984 _RS21420 BPHYT_RS21425: BPHYT_RS21425 - 3-carboxy-cis,cis-muconate cycloisomerase, at 365,077 to 366,456 _RS21425 BPHYT_RS21430: BPHYT_RS21430 - 3-oxoadipate enol-lactonase, at 366,462 to 367,253 _RS21430 BPHYT_RS21435: BPHYT_RS21435 - 4-carboxymuconolactone decarboxylase, at 367,250 to 367,645 _RS21435 Position (kb) 365 366 367Strain fitness (log2 ratio) -1 0 1at 364.112 kb on - strand, within BPHYT_RS21415at 364.388 kb on + strandat 364.414 kb on + strand, within BPHYT_RS21420at 364.571 kb on + strand, within BPHYT_RS21420at 364.748 kb on + strand, within BPHYT_RS21420at 364.844 kb on + strand, within BPHYT_RS21420at 364.845 kb on - strand, within BPHYT_RS21420at 365.038 kb on + strandat 365.038 kb on + strandat 365.381 kb on - strand, within BPHYT_RS21425at 366.373 kb on + strandat 366.373 kb on + strandat 366.374 kb on - strandat 366.374 kb on - strandat 366.394 kb on + strandat 366.394 kb on + strandat 366.469 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10
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364,112 - BPHYT_RS21415 0.72 -0.8
364,388 + -1.4
364,414 + BPHYT_RS21420 0.13 +0.7
364,571 + BPHYT_RS21420 0.37 +0.3
364,748 + BPHYT_RS21420 0.64 +0.5
364,844 + BPHYT_RS21420 0.79 +0.4
364,845 - BPHYT_RS21420 0.79 +0.7
365,038 + -0.8
365,038 + -0.8
365,381 - BPHYT_RS21425 0.22 -1.6
366,373 + -0.1
366,373 + -0.0
366,374 - +1.7
366,374 - -0.3
366,394 + +0.2
366,394 + -0.0
366,469 - +0.1

Or see this region's nucleotide sequence