Experiment: 36hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21415 and BPHYT_RS21420 are separated by 13 nucleotides BPHYT_RS21420 and BPHYT_RS21425 are separated by 92 nucleotides BPHYT_RS21425 and BPHYT_RS21430 are separated by 5 nucleotides BPHYT_RS21430 and BPHYT_RS21435 overlap by 4 nucleotides
BPHYT_RS21415: BPHYT_RS21415 - 3-oxoadipate CoA-transferase subunit A, at 363,604 to 364,311
_RS21415
BPHYT_RS21420: BPHYT_RS21420 - 3-oxoadipate CoA-transferase subunit B, at 364,325 to 364,984
_RS21420
BPHYT_RS21425: BPHYT_RS21425 - 3-carboxy-cis,cis-muconate cycloisomerase, at 365,077 to 366,456
_RS21425
BPHYT_RS21430: BPHYT_RS21430 - 3-oxoadipate enol-lactonase, at 366,462 to 367,253
_RS21430
BPHYT_RS21435: BPHYT_RS21435 - 4-carboxymuconolactone decarboxylase, at 367,250 to 367,645
_RS21435
Position (kb) 365
366
367 Strain fitness (log2 ratio) -1
0
1 at 364.112 kb on - strand, within BPHYT_RS21415 at 364.388 kb on + strand at 364.414 kb on + strand, within BPHYT_RS21420 at 364.571 kb on + strand, within BPHYT_RS21420 at 364.748 kb on + strand, within BPHYT_RS21420 at 364.844 kb on + strand, within BPHYT_RS21420 at 364.845 kb on - strand, within BPHYT_RS21420 at 365.038 kb on + strand at 365.038 kb on + strand at 365.381 kb on - strand, within BPHYT_RS21425 at 366.373 kb on + strand at 366.373 kb on + strand at 366.374 kb on - strand at 366.374 kb on - strand at 366.394 kb on + strand at 366.394 kb on + strand at 366.469 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10 remove 364,112 - BPHYT_RS21415 0.72 -0.8 364,388 + -1.4 364,414 + BPHYT_RS21420 0.13 +0.7 364,571 + BPHYT_RS21420 0.37 +0.3 364,748 + BPHYT_RS21420 0.64 +0.5 364,844 + BPHYT_RS21420 0.79 +0.4 364,845 - BPHYT_RS21420 0.79 +0.7 365,038 + -0.8 365,038 + -0.8 365,381 - BPHYT_RS21425 0.22 -1.6 366,373 + -0.1 366,373 + -0.0 366,374 - +1.7 366,374 - -0.3 366,394 + +0.2 366,394 + -0.0 366,469 - +0.1
Or see this region's nucleotide sequence