Experiment: 36hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20000 and BPHYT_RS20005 are separated by 353 nucleotides BPHYT_RS20005 and BPHYT_RS20010 are separated by 10 nucleotides
BPHYT_RS20000: BPHYT_RS20000 - serine/threonine protein kinase, at 29,770 to 31,245
_RS20000
BPHYT_RS20005: BPHYT_RS20005 - chlorogenate esterase, at 31,599 to 33,332
_RS20005
BPHYT_RS20010: BPHYT_RS20010 - membrane protein, at 33,343 to 34,482
_RS20010
Position (kb) 31
32
33
34 Strain fitness (log2 ratio) -2
-1
0
1 at 31.052 kb on + strand, within BPHYT_RS20000 at 31.052 kb on + strand, within BPHYT_RS20000 at 31.142 kb on + strand at 31.299 kb on + strand at 31.382 kb on + strand at 31.382 kb on + strand at 31.383 kb on - strand at 31.537 kb on - strand at 31.537 kb on - strand at 31.919 kb on - strand, within BPHYT_RS20005 at 31.969 kb on + strand, within BPHYT_RS20005 at 31.970 kb on - strand, within BPHYT_RS20005 at 32.263 kb on + strand, within BPHYT_RS20005 at 32.263 kb on + strand, within BPHYT_RS20005 at 32.264 kb on - strand, within BPHYT_RS20005 at 32.639 kb on - strand, within BPHYT_RS20005 at 32.963 kb on - strand, within BPHYT_RS20005 at 33.458 kb on + strand, within BPHYT_RS20010 at 33.501 kb on - strand, within BPHYT_RS20010 at 33.560 kb on + strand, within BPHYT_RS20010 at 33.561 kb on - strand, within BPHYT_RS20010 at 33.998 kb on + strand, within BPHYT_RS20010 at 34.046 kb on + strand, within BPHYT_RS20010 at 34.070 kb on + strand, within BPHYT_RS20010 at 34.112 kb on + strand, within BPHYT_RS20010
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10 remove 31,052 + BPHYT_RS20000 0.87 -0.1 31,052 + BPHYT_RS20000 0.87 -0.7 31,142 + +0.2 31,299 + -1.1 31,382 + +1.2 31,382 + +0.2 31,383 - -2.0 31,537 - -0.3 31,537 - +0.2 31,919 - BPHYT_RS20005 0.18 +0.2 31,969 + BPHYT_RS20005 0.21 +0.3 31,970 - BPHYT_RS20005 0.21 -1.7 32,263 + BPHYT_RS20005 0.38 -0.7 32,263 + BPHYT_RS20005 0.38 -0.9 32,264 - BPHYT_RS20005 0.38 -0.3 32,639 - BPHYT_RS20005 0.60 +0.0 32,963 - BPHYT_RS20005 0.79 +0.1 33,458 + BPHYT_RS20010 0.10 +0.3 33,501 - BPHYT_RS20010 0.14 +1.0 33,560 + BPHYT_RS20010 0.19 -1.0 33,561 - BPHYT_RS20010 0.19 +0.9 33,998 + BPHYT_RS20010 0.57 +0.3 34,046 + BPHYT_RS20010 0.62 +0.7 34,070 + BPHYT_RS20010 0.64 -0.7 34,112 + BPHYT_RS20010 0.67 +0.1
Or see this region's nucleotide sequence