Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS19755

Experiment: 36hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS19745 and BPHYT_RS19750 are separated by 105 nucleotidesBPHYT_RS19750 and BPHYT_RS19755 are separated by 241 nucleotidesBPHYT_RS19755 and BPHYT_RS19760 are separated by 68 nucleotides BPHYT_RS19745: BPHYT_RS19745 - membrane protein, at 4,443,369 to 4,443,548 _RS19745 BPHYT_RS19750: BPHYT_RS19750 - hypothetical protein, at 4,443,654 to 4,443,851 _RS19750 BPHYT_RS19755: BPHYT_RS19755 - HIT family hydrolase, at 4,444,093 to 4,444,518 _RS19755 BPHYT_RS19760: BPHYT_RS19760 - AraC family transcriptional regulator, at 4,444,587 to 4,445,528 _RS19760 Position (kb) 4444 4445Strain fitness (log2 ratio) -2 -1 0 1 2at 4443.152 kb on + strandat 4443.211 kb on - strandat 4443.215 kb on + strandat 4443.216 kb on - strandat 4443.220 kb on + strandat 4443.220 kb on + strandat 4443.220 kb on + strandat 4443.220 kb on + strandat 4443.334 kb on - strandat 4443.617 kb on - strandat 4444.043 kb on + strandat 4444.043 kb on + strandat 4444.044 kb on - strandat 4444.044 kb on - strandat 4444.044 kb on - strandat 4444.511 kb on + strandat 4444.589 kb on - strandat 4444.976 kb on - strand, within BPHYT_RS19760at 4444.976 kb on - strand, within BPHYT_RS19760at 4445.100 kb on - strand, within BPHYT_RS19760at 4445.335 kb on + strand, within BPHYT_RS19760

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10
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4,443,152 + +0.6
4,443,211 - +0.9
4,443,215 + -0.2
4,443,216 - -1.1
4,443,220 + +0.8
4,443,220 + +0.6
4,443,220 + -1.2
4,443,220 + +1.4
4,443,334 - -0.9
4,443,617 - +0.6
4,444,043 + -0.3
4,444,043 + +0.2
4,444,044 - +0.3
4,444,044 - +0.5
4,444,044 - -1.9
4,444,511 + -1.0
4,444,589 - +0.1
4,444,976 - BPHYT_RS19760 0.41 -0.1
4,444,976 - BPHYT_RS19760 0.41 +1.9
4,445,100 - BPHYT_RS19760 0.54 -0.1
4,445,335 + BPHYT_RS19760 0.79 +1.1

Or see this region's nucleotide sequence