Experiment: 36hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13505 and BPHYT_RS13510 are separated by 232 nucleotides BPHYT_RS13510 and BPHYT_RS13515 are separated by 100 nucleotides BPHYT_RS13515 and BPHYT_RS13520 overlap by 4 nucleotides
BPHYT_RS13505: BPHYT_RS13505 - 2,4-dienoyl-CoA reductase, at 3,081,345 to 3,082,265
_RS13505
BPHYT_RS13510: BPHYT_RS13510 - C4-dicarboxylate transporter, at 3,082,498 to 3,083,745
_RS13510
BPHYT_RS13515: BPHYT_RS13515 - 3-isopropylmalate dehydratase small subunit, at 3,083,846 to 3,084,457
_RS13515
BPHYT_RS13520: BPHYT_RS13520 - isopropylmalate isomerase, at 3,084,454 to 3,085,869
_RS13520
Position (kb)
3082
3083
3084 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3081.569 kb on + strand, within BPHYT_RS13505 at 3081.588 kb on + strand, within BPHYT_RS13505 at 3081.611 kb on - strand, within BPHYT_RS13505 at 3081.815 kb on + strand, within BPHYT_RS13505 at 3081.817 kb on + strand, within BPHYT_RS13505 at 3081.818 kb on - strand, within BPHYT_RS13505 at 3081.818 kb on - strand, within BPHYT_RS13505 at 3082.312 kb on - strand at 3082.818 kb on - strand, within BPHYT_RS13510 at 3082.821 kb on + strand, within BPHYT_RS13510 at 3082.822 kb on - strand, within BPHYT_RS13510 at 3082.977 kb on - strand, within BPHYT_RS13510 at 3082.977 kb on - strand, within BPHYT_RS13510 at 3082.977 kb on - strand, within BPHYT_RS13510 at 3083.194 kb on - strand, within BPHYT_RS13510 at 3083.385 kb on - strand, within BPHYT_RS13510 at 3083.562 kb on + strand, within BPHYT_RS13510 at 3083.715 kb on - strand at 3083.715 kb on - strand at 3083.727 kb on - strand at 3083.847 kb on + strand at 3083.848 kb on - strand at 3084.267 kb on + strand, within BPHYT_RS13515 at 3084.268 kb on - strand, within BPHYT_RS13515 at 3084.331 kb on - strand at 3084.331 kb on - strand, within BPHYT_RS13515
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10 remove 3,081,569 + BPHYT_RS13505 0.24 -0.0 3,081,588 + BPHYT_RS13505 0.26 +0.7 3,081,611 - BPHYT_RS13505 0.29 +1.4 3,081,815 + BPHYT_RS13505 0.51 +0.9 3,081,817 + BPHYT_RS13505 0.51 -0.2 3,081,818 - BPHYT_RS13505 0.51 -0.2 3,081,818 - BPHYT_RS13505 0.51 +0.7 3,082,312 - -0.2 3,082,818 - BPHYT_RS13510 0.26 -0.0 3,082,821 + BPHYT_RS13510 0.26 +0.5 3,082,822 - BPHYT_RS13510 0.26 +1.4 3,082,977 - BPHYT_RS13510 0.38 -0.2 3,082,977 - BPHYT_RS13510 0.38 -1.6 3,082,977 - BPHYT_RS13510 0.38 -0.3 3,083,194 - BPHYT_RS13510 0.56 +0.3 3,083,385 - BPHYT_RS13510 0.71 -0.1 3,083,562 + BPHYT_RS13510 0.85 -0.3 3,083,715 - -1.0 3,083,715 - -1.6 3,083,727 - -3.5 3,083,847 + -0.3 3,083,848 - -1.0 3,084,267 + BPHYT_RS13515 0.69 -1.8 3,084,268 - BPHYT_RS13515 0.69 -1.2 3,084,331 - -0.7 3,084,331 - BPHYT_RS13515 0.79 -1.4
Or see this region's nucleotide sequence