Experiment: 36hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11530 and BPHYT_RS11535 are separated by 338 nucleotides BPHYT_RS11535 and BPHYT_RS11540 are separated by 435 nucleotides BPHYT_RS11540 and BPHYT_RS11545 overlap by 4 nucleotides
BPHYT_RS11530: BPHYT_RS11530 - 5-hydroxyisourate hydrolase, at 2,621,977 to 2,622,330
_RS11530
BPHYT_RS11535: BPHYT_RS11535 - membrane protein, at 2,622,669 to 2,623,862
_RS11535
BPHYT_RS11540: BPHYT_RS11540 - ureidoglycolate hydrolase, at 2,624,298 to 2,624,810
_RS11540
BPHYT_RS11545: BPHYT_RS11545 - allantoicase, at 2,624,807 to 2,625,820
_RS11545
Position (kb)
2622
2623
2624 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2621.689 kb on + strand at 2621.689 kb on + strand at 2621.689 kb on + strand at 2621.741 kb on - strand at 2621.799 kb on + strand at 2621.799 kb on + strand at 2621.800 kb on - strand at 2621.844 kb on - strand at 2621.885 kb on - strand at 2621.925 kb on - strand at 2622.274 kb on + strand, within BPHYT_RS11530 at 2622.282 kb on - strand, within BPHYT_RS11530 at 2622.307 kb on + strand at 2622.308 kb on - strand at 2622.627 kb on - strand at 2622.681 kb on - strand at 2622.993 kb on + strand, within BPHYT_RS11535 at 2623.081 kb on - strand, within BPHYT_RS11535 at 2623.143 kb on + strand, within BPHYT_RS11535 at 2623.422 kb on + strand, within BPHYT_RS11535 at 2623.753 kb on - strand at 2623.860 kb on + strand at 2623.861 kb on - strand at 2623.985 kb on - strand at 2624.020 kb on - strand at 2624.080 kb on + strand at 2624.081 kb on - strand at 2624.127 kb on + strand at 2624.129 kb on + strand at 2624.130 kb on - strand at 2624.130 kb on - strand at 2624.130 kb on - strand at 2624.212 kb on + strand at 2624.363 kb on + strand, within BPHYT_RS11540 at 2624.400 kb on - strand, within BPHYT_RS11540 at 2624.539 kb on + strand, within BPHYT_RS11540 at 2624.540 kb on - strand, within BPHYT_RS11540
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10 remove 2,621,689 + +0.0 2,621,689 + -0.2 2,621,689 + +0.2 2,621,741 - +0.2 2,621,799 + -0.3 2,621,799 + -0.6 2,621,800 - -1.0 2,621,844 - -1.0 2,621,885 - -0.3 2,621,925 - -0.0 2,622,274 + BPHYT_RS11530 0.84 -1.0 2,622,282 - BPHYT_RS11530 0.86 -1.1 2,622,307 + -1.8 2,622,308 - -0.3 2,622,627 - +0.1 2,622,681 - +0.5 2,622,993 + BPHYT_RS11535 0.27 -0.1 2,623,081 - BPHYT_RS11535 0.35 +1.6 2,623,143 + BPHYT_RS11535 0.40 -1.5 2,623,422 + BPHYT_RS11535 0.63 +0.3 2,623,753 - -1.3 2,623,860 + +1.4 2,623,861 - -0.2 2,623,985 - -1.5 2,624,020 - -0.8 2,624,080 + -0.7 2,624,081 - +3.5 2,624,127 + +0.7 2,624,129 + -0.4 2,624,130 - -2.0 2,624,130 - -1.3 2,624,130 - +1.1 2,624,212 + +0.7 2,624,363 + BPHYT_RS11540 0.13 +0.3 2,624,400 - BPHYT_RS11540 0.20 -0.7 2,624,539 + BPHYT_RS11540 0.47 -1.5 2,624,540 - BPHYT_RS11540 0.47 -1.5
Or see this region's nucleotide sequence