Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34015

Experiment: 36hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34005 and BPHYT_RS34010 are separated by 612 nucleotidesBPHYT_RS34010 and BPHYT_RS34015 are separated by 25 nucleotidesBPHYT_RS34015 and BPHYT_RS34020 overlap by 1 nucleotidesBPHYT_RS34020 and BPHYT_RS34025 overlap by 4 nucleotides BPHYT_RS34005: BPHYT_RS34005 - hemagglutinin, at 3,221,291 to 3,229,789 _RS34005 BPHYT_RS34010: BPHYT_RS34010 - pilus assembly protein, at 3,230,402 to 3,230,683 _RS34010 BPHYT_RS34015: BPHYT_RS34015 - hypothetical protein, at 3,230,709 to 3,232,235 _RS34015 BPHYT_RS34020: BPHYT_RS34020 - hypothetical protein, at 3,232,235 to 3,233,119 _RS34020 BPHYT_RS34025: BPHYT_RS34025 - hypothetical protein, at 3,233,116 to 3,233,835 _RS34025 Position (kb) 3230 3231 3232 3233Strain fitness (log2 ratio) -2 -1 0 1 2at 3229.777 kb on - strandat 3230.102 kb on + strandat 3230.263 kb on - strandat 3230.268 kb on + strandat 3230.459 kb on + strand, within BPHYT_RS34010at 3230.459 kb on + strand, within BPHYT_RS34010at 3230.459 kb on + strand, within BPHYT_RS34010at 3230.459 kb on + strand, within BPHYT_RS34010at 3230.459 kb on + strand, within BPHYT_RS34010at 3230.459 kb on + strand, within BPHYT_RS34010at 3230.602 kb on + strand, within BPHYT_RS34010at 3230.655 kb on - strandat 3230.655 kb on - strandat 3230.655 kb on - strandat 3230.656 kb on + strandat 3230.885 kb on + strand, within BPHYT_RS34015at 3230.914 kb on - strand, within BPHYT_RS34015at 3231.016 kb on - strand, within BPHYT_RS34015at 3231.022 kb on + strand, within BPHYT_RS34015at 3231.070 kb on + strand, within BPHYT_RS34015at 3231.337 kb on - strand, within BPHYT_RS34015at 3231.506 kb on + strand, within BPHYT_RS34015at 3231.507 kb on - strand, within BPHYT_RS34015at 3231.507 kb on - strand, within BPHYT_RS34015at 3231.507 kb on - strand, within BPHYT_RS34015at 3231.675 kb on + strand, within BPHYT_RS34015at 3231.676 kb on - strand, within BPHYT_RS34015at 3231.677 kb on + strand, within BPHYT_RS34015at 3231.720 kb on + strand, within BPHYT_RS34015at 3231.853 kb on - strand, within BPHYT_RS34015at 3231.921 kb on - strand, within BPHYT_RS34015at 3232.249 kb on + strandat 3232.249 kb on + strandat 3232.484 kb on + strand, within BPHYT_RS34020at 3232.485 kb on - strand, within BPHYT_RS34020at 3232.507 kb on - strand, within BPHYT_RS34020at 3232.507 kb on - strand, within BPHYT_RS34020at 3232.721 kb on - strand, within BPHYT_RS34020at 3232.721 kb on - strand, within BPHYT_RS34020at 3232.934 kb on - strand, within BPHYT_RS34020at 3233.025 kb on - strand, within BPHYT_RS34020at 3233.091 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10
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3,229,777 - -0.3
3,230,102 + -0.5
3,230,263 - +1.5
3,230,268 + +1.0
3,230,459 + BPHYT_RS34010 0.20 -0.1
3,230,459 + BPHYT_RS34010 0.20 +0.2
3,230,459 + BPHYT_RS34010 0.20 -2.4
3,230,459 + BPHYT_RS34010 0.20 -1.4
3,230,459 + BPHYT_RS34010 0.20 -1.2
3,230,459 + BPHYT_RS34010 0.20 -0.4
3,230,602 + BPHYT_RS34010 0.71 +0.2
3,230,655 - -0.2
3,230,655 - -1.2
3,230,655 - +0.5
3,230,656 + -0.7
3,230,885 + BPHYT_RS34015 0.12 -1.3
3,230,914 - BPHYT_RS34015 0.13 +0.9
3,231,016 - BPHYT_RS34015 0.20 +0.2
3,231,022 + BPHYT_RS34015 0.20 -0.6
3,231,070 + BPHYT_RS34015 0.24 +0.3
3,231,337 - BPHYT_RS34015 0.41 +2.3
3,231,506 + BPHYT_RS34015 0.52 -0.0
3,231,507 - BPHYT_RS34015 0.52 -0.3
3,231,507 - BPHYT_RS34015 0.52 +0.3
3,231,507 - BPHYT_RS34015 0.52 -1.1
3,231,675 + BPHYT_RS34015 0.63 -0.2
3,231,676 - BPHYT_RS34015 0.63 -0.4
3,231,677 + BPHYT_RS34015 0.63 -1.2
3,231,720 + BPHYT_RS34015 0.66 -0.2
3,231,853 - BPHYT_RS34015 0.75 -1.6
3,231,921 - BPHYT_RS34015 0.79 -0.6
3,232,249 + +0.3
3,232,249 + -1.3
3,232,484 + BPHYT_RS34020 0.28 -1.3
3,232,485 - BPHYT_RS34020 0.28 -0.7
3,232,507 - BPHYT_RS34020 0.31 -0.7
3,232,507 - BPHYT_RS34020 0.31 +1.4
3,232,721 - BPHYT_RS34020 0.55 -0.9
3,232,721 - BPHYT_RS34020 0.55 -1.0
3,232,934 - BPHYT_RS34020 0.79 -0.9
3,233,025 - BPHYT_RS34020 0.89 -0.7
3,233,091 + -1.7

Or see this region's nucleotide sequence