Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31100

Experiment: 36hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31090 and BPHYT_RS31095 are separated by 101 nucleotidesBPHYT_RS31095 and BPHYT_RS31100 are separated by 536 nucleotidesBPHYT_RS31100 and BPHYT_RS31105 are separated by 431 nucleotides BPHYT_RS31090: BPHYT_RS31090 - hypothetical protein, at 2,530,791 to 2,532,107 _RS31090 BPHYT_RS31095: BPHYT_RS31095 - hypothetical protein, at 2,532,209 to 2,532,394 _RS31095 BPHYT_RS31100: BPHYT_RS31100 - AraC family transcriptional regulator, at 2,532,931 to 2,533,923 _RS31100 BPHYT_RS31105: BPHYT_RS31105 - UTP--glucose-1-phosphate uridylyltransferase, at 2,534,355 to 2,535,236 _RS31105 Position (kb) 2532 2533 2534Strain fitness (log2 ratio) -2 -1 0 1at 2532.035 kb on + strandat 2532.556 kb on + strandat 2532.853 kb on + strandat 2532.942 kb on - strandat 2532.945 kb on + strandat 2533.227 kb on - strand, within BPHYT_RS31100at 2533.376 kb on - strand, within BPHYT_RS31100at 2533.376 kb on - strand, within BPHYT_RS31100at 2533.388 kb on + strand, within BPHYT_RS31100at 2533.389 kb on - strand, within BPHYT_RS31100at 2533.389 kb on - strand, within BPHYT_RS31100at 2533.753 kb on - strand, within BPHYT_RS31100at 2534.201 kb on - strandat 2534.259 kb on - strandat 2534.378 kb on - strandat 2534.865 kb on + strand, within BPHYT_RS31105

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10
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2,532,035 + -0.7
2,532,556 + -0.5
2,532,853 + -1.9
2,532,942 - +0.9
2,532,945 + +0.3
2,533,227 - BPHYT_RS31100 0.30 +0.0
2,533,376 - BPHYT_RS31100 0.45 -1.7
2,533,376 - BPHYT_RS31100 0.45 -0.7
2,533,388 + BPHYT_RS31100 0.46 -0.9
2,533,389 - BPHYT_RS31100 0.46 +0.4
2,533,389 - BPHYT_RS31100 0.46 +1.0
2,533,753 - BPHYT_RS31100 0.83 +0.4
2,534,201 - +0.3
2,534,259 - +0.4
2,534,378 - -0.4
2,534,865 + BPHYT_RS31105 0.58 +0.1

Or see this region's nucleotide sequence