Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS30200

Experiment: 36hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS30195 and BPHYT_RS30200 are separated by 79 nucleotidesBPHYT_RS30200 and BPHYT_RS30205 are separated by 43 nucleotides BPHYT_RS30195: BPHYT_RS30195 - ABC transporter substrate-binding protein, at 2,328,450 to 2,330,066 _RS30195 BPHYT_RS30200: BPHYT_RS30200 - FAD-dependent oxidoreductase, at 2,330,146 to 2,331,420 _RS30200 BPHYT_RS30205: BPHYT_RS30205 - dehalogenase, at 2,331,464 to 2,332,132 _RS30205 Position (kb) 2330 2331 2332Strain fitness (log2 ratio) -2 -1 0 1at 2329.778 kb on - strand, within BPHYT_RS30195at 2329.799 kb on - strand, within BPHYT_RS30195at 2330.174 kb on + strandat 2330.174 kb on + strandat 2330.175 kb on - strandat 2330.345 kb on + strand, within BPHYT_RS30200at 2330.414 kb on + strand, within BPHYT_RS30200at 2331.069 kb on - strand, within BPHYT_RS30200at 2331.069 kb on - strand, within BPHYT_RS30200at 2331.465 kb on + strandat 2331.510 kb on + strandat 2331.514 kb on - strandat 2331.519 kb on + strandat 2331.522 kb on + strandat 2331.523 kb on - strandat 2331.663 kb on + strand, within BPHYT_RS30205at 2331.663 kb on + strand, within BPHYT_RS30205at 2331.722 kb on - strand, within BPHYT_RS30205at 2331.798 kb on + strand, within BPHYT_RS30205at 2332.111 kb on - strandat 2332.111 kb on - strandat 2332.230 kb on + strandat 2332.383 kb on + strandat 2332.383 kb on + strandat 2332.384 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10
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2,329,778 - BPHYT_RS30195 0.82 +0.3
2,329,799 - BPHYT_RS30195 0.83 +0.2
2,330,174 + -0.1
2,330,174 + -1.0
2,330,175 - -0.1
2,330,345 + BPHYT_RS30200 0.16 +0.1
2,330,414 + BPHYT_RS30200 0.21 +0.2
2,331,069 - BPHYT_RS30200 0.72 -0.9
2,331,069 - BPHYT_RS30200 0.72 -1.9
2,331,465 + +1.2
2,331,510 + +0.7
2,331,514 - -0.4
2,331,519 + +0.5
2,331,522 + -0.2
2,331,523 - -0.0
2,331,663 + BPHYT_RS30205 0.30 +0.4
2,331,663 + BPHYT_RS30205 0.30 -0.3
2,331,722 - BPHYT_RS30205 0.39 -0.4
2,331,798 + BPHYT_RS30205 0.50 -0.5
2,332,111 - -1.0
2,332,111 - +0.7
2,332,230 + -0.2
2,332,383 + +0.2
2,332,383 + +0.1
2,332,384 - +0.1

Or see this region's nucleotide sequence