Experiment: 36hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29400 and BPHYT_RS29405 are separated by 87 nucleotides BPHYT_RS29405 and BPHYT_RS29410 overlap by 4 nucleotides BPHYT_RS29410 and BPHYT_RS29415 are separated by 13 nucleotides BPHYT_RS29415 and BPHYT_RS29420 are separated by 66 nucleotides
BPHYT_RS29400: BPHYT_RS29400 - MFS transporter, at 2,151,099 to 2,152,436
_RS29400
BPHYT_RS29405: BPHYT_RS29405 - aldolase, at 2,152,524 to 2,153,186
_RS29405
BPHYT_RS29410: BPHYT_RS29410 - HPr kinase, at 2,153,183 to 2,154,493
_RS29410
BPHYT_RS29415: BPHYT_RS29415 - 3-hydroxyisobutyrate dehydrogenase, at 2,154,507 to 2,155,397
_RS29415
BPHYT_RS29420: BPHYT_RS29420 - GntR family transcriptional regulator, at 2,155,464 to 2,156,168
_RS29420
Position (kb)
2153
2154
2155 Strain fitness (log2 ratio)
-1
0
1
2 at 2152.306 kb on + strand at 2152.349 kb on + strand at 2152.426 kb on + strand at 2152.918 kb on + strand, within BPHYT_RS29405 at 2153.842 kb on - strand, within BPHYT_RS29410 at 2154.229 kb on - strand, within BPHYT_RS29410 at 2154.394 kb on + strand at 2154.394 kb on + strand at 2154.484 kb on + strand at 2154.484 kb on + strand at 2154.710 kb on - strand, within BPHYT_RS29415 at 2155.408 kb on + strand at 2155.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10 remove 2,152,306 + -0.9 2,152,349 + +0.2 2,152,426 + -0.6 2,152,918 + BPHYT_RS29405 0.59 +2.0 2,153,842 - BPHYT_RS29410 0.50 +0.8 2,154,229 - BPHYT_RS29410 0.80 -1.0 2,154,394 + +0.6 2,154,394 + -1.1 2,154,484 + -0.1 2,154,484 + -1.4 2,154,710 - BPHYT_RS29415 0.23 -1.5 2,155,408 + -1.1 2,155,409 - +0.1
Or see this region's nucleotide sequence