Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29410

Experiment: 36hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29400 and BPHYT_RS29405 are separated by 87 nucleotidesBPHYT_RS29405 and BPHYT_RS29410 overlap by 4 nucleotidesBPHYT_RS29410 and BPHYT_RS29415 are separated by 13 nucleotidesBPHYT_RS29415 and BPHYT_RS29420 are separated by 66 nucleotides BPHYT_RS29400: BPHYT_RS29400 - MFS transporter, at 2,151,099 to 2,152,436 _RS29400 BPHYT_RS29405: BPHYT_RS29405 - aldolase, at 2,152,524 to 2,153,186 _RS29405 BPHYT_RS29410: BPHYT_RS29410 - HPr kinase, at 2,153,183 to 2,154,493 _RS29410 BPHYT_RS29415: BPHYT_RS29415 - 3-hydroxyisobutyrate dehydrogenase, at 2,154,507 to 2,155,397 _RS29415 BPHYT_RS29420: BPHYT_RS29420 - GntR family transcriptional regulator, at 2,155,464 to 2,156,168 _RS29420 Position (kb) 2153 2154 2155Strain fitness (log2 ratio) -1 0 1 2at 2152.306 kb on + strandat 2152.349 kb on + strandat 2152.426 kb on + strandat 2152.918 kb on + strand, within BPHYT_RS29405at 2153.842 kb on - strand, within BPHYT_RS29410at 2154.229 kb on - strand, within BPHYT_RS29410at 2154.394 kb on + strandat 2154.394 kb on + strandat 2154.484 kb on + strandat 2154.484 kb on + strandat 2154.710 kb on - strand, within BPHYT_RS29415at 2155.408 kb on + strandat 2155.409 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10
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2,152,306 + -0.9
2,152,349 + +0.2
2,152,426 + -0.6
2,152,918 + BPHYT_RS29405 0.59 +2.0
2,153,842 - BPHYT_RS29410 0.50 +0.8
2,154,229 - BPHYT_RS29410 0.80 -1.0
2,154,394 + +0.6
2,154,394 + -1.1
2,154,484 + -0.1
2,154,484 + -1.4
2,154,710 - BPHYT_RS29415 0.23 -1.5
2,155,408 + -1.1
2,155,409 - +0.1

Or see this region's nucleotide sequence