Experiment: 36hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25155 and BPHYT_RS25160 are separated by 11 nucleotides BPHYT_RS25160 and BPHYT_RS25165 are separated by 42 nucleotides BPHYT_RS25165 and BPHYT_RS25170 are separated by 283 nucleotides
BPHYT_RS25155: BPHYT_RS25155 - oxygen-dependent choline dehydrogenase, at 1,215,025 to 1,216,710
_RS25155
BPHYT_RS25160: BPHYT_RS25160 - NAD/NADP-dependent betaine aldehyde dehydrogenase, at 1,216,722 to 1,218,191
_RS25160
BPHYT_RS25165: BPHYT_RS25165 - HTH-type transcriptional regulator BetI, at 1,218,234 to 1,218,821
_RS25165
BPHYT_RS25170: BPHYT_RS25170 - aldehyde dismutase, at 1,219,105 to 1,220,304
_RS25170
Position (kb)
1216
1217
1218
1219 Strain fitness (log2 ratio)
-2
-1
0
1 at 1216.258 kb on - strand, within BPHYT_RS25155 at 1216.349 kb on + strand, within BPHYT_RS25155 at 1216.415 kb on + strand, within BPHYT_RS25155 at 1216.488 kb on + strand, within BPHYT_RS25155 at 1216.689 kb on - strand at 1216.869 kb on - strand, within BPHYT_RS25160 at 1216.869 kb on - strand, within BPHYT_RS25160 at 1217.845 kb on + strand, within BPHYT_RS25160 at 1218.164 kb on - strand at 1218.164 kb on - strand at 1218.264 kb on - strand at 1218.446 kb on - strand, within BPHYT_RS25165 at 1218.446 kb on - strand, within BPHYT_RS25165 at 1218.834 kb on - strand at 1218.836 kb on - strand at 1219.129 kb on + strand at 1219.130 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10 remove 1,216,258 - BPHYT_RS25155 0.73 -0.7 1,216,349 + BPHYT_RS25155 0.79 +0.8 1,216,415 + BPHYT_RS25155 0.82 +1.1 1,216,488 + BPHYT_RS25155 0.87 -0.2 1,216,689 - +0.1 1,216,869 - BPHYT_RS25160 0.10 -0.6 1,216,869 - BPHYT_RS25160 0.10 +0.6 1,217,845 + BPHYT_RS25160 0.76 +1.2 1,218,164 - +0.9 1,218,164 - +0.3 1,218,264 - +0.1 1,218,446 - BPHYT_RS25165 0.36 -1.0 1,218,446 - BPHYT_RS25165 0.36 -2.7 1,218,834 - +0.4 1,218,836 - -1.0 1,219,129 + -2.0 1,219,130 - +0.1
Or see this region's nucleotide sequence