Experiment: 36hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS24370 and BPHYT_RS24375 are separated by 73 nucleotides BPHYT_RS24375 and BPHYT_RS24380 overlap by 4 nucleotides
BPHYT_RS24370: BPHYT_RS24370 - glucose-6-phosphate 1-dehydrogenase, at 1,032,663 to 1,034,144
_RS24370
BPHYT_RS24375: BPHYT_RS24375 - type VI secretion system protein ImpK, at 1,034,218 to 1,035,000
_RS24375
BPHYT_RS24380: BPHYT_RS24380 - type VI secretion protein, at 1,034,997 to 1,036,343
_RS24380
Position (kb)
1034
1035
1036 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1033.353 kb on + strand, within BPHYT_RS24370 at 1033.353 kb on + strand, within BPHYT_RS24370 at 1033.354 kb on - strand, within BPHYT_RS24370 at 1033.359 kb on - strand at 1033.359 kb on - strand, within BPHYT_RS24370 at 1033.643 kb on + strand, within BPHYT_RS24370 at 1033.863 kb on - strand, within BPHYT_RS24370 at 1033.927 kb on - strand, within BPHYT_RS24370 at 1034.063 kb on + strand at 1034.142 kb on + strand at 1034.142 kb on + strand at 1034.277 kb on - strand at 1034.312 kb on + strand, within BPHYT_RS24375 at 1034.316 kb on + strand, within BPHYT_RS24375 at 1034.317 kb on - strand, within BPHYT_RS24375 at 1034.331 kb on - strand, within BPHYT_RS24375 at 1034.753 kb on + strand, within BPHYT_RS24375 at 1034.753 kb on + strand, within BPHYT_RS24375 at 1034.925 kb on - strand at 1034.993 kb on + strand at 1034.994 kb on - strand at 1035.052 kb on + strand at 1035.053 kb on - strand at 1035.053 kb on - strand at 1035.081 kb on - strand at 1035.081 kb on - strand at 1035.276 kb on - strand, within BPHYT_RS24380 at 1035.295 kb on + strand, within BPHYT_RS24380 at 1035.296 kb on - strand, within BPHYT_RS24380 at 1035.575 kb on - strand, within BPHYT_RS24380 at 1035.688 kb on + strand, within BPHYT_RS24380 at 1035.789 kb on - strand, within BPHYT_RS24380 at 1035.960 kb on + strand, within BPHYT_RS24380 at 1035.964 kb on + strand, within BPHYT_RS24380
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10 remove 1,033,353 + BPHYT_RS24370 0.47 +1.2 1,033,353 + BPHYT_RS24370 0.47 -0.2 1,033,354 - BPHYT_RS24370 0.47 -1.4 1,033,359 - +0.6 1,033,359 - BPHYT_RS24370 0.47 +0.6 1,033,643 + BPHYT_RS24370 0.66 -0.4 1,033,863 - BPHYT_RS24370 0.81 +0.4 1,033,927 - BPHYT_RS24370 0.85 -0.8 1,034,063 + -2.9 1,034,142 + -0.5 1,034,142 + -0.9 1,034,277 - -0.4 1,034,312 + BPHYT_RS24375 0.12 +0.4 1,034,316 + BPHYT_RS24375 0.13 -0.1 1,034,317 - BPHYT_RS24375 0.13 -0.8 1,034,331 - BPHYT_RS24375 0.14 -0.1 1,034,753 + BPHYT_RS24375 0.68 -1.4 1,034,753 + BPHYT_RS24375 0.68 -0.8 1,034,925 - -0.1 1,034,993 + -1.4 1,034,994 - -0.9 1,035,052 + +0.4 1,035,053 - +0.8 1,035,053 - +0.4 1,035,081 - +2.2 1,035,081 - +0.4 1,035,276 - BPHYT_RS24380 0.21 -2.3 1,035,295 + BPHYT_RS24380 0.22 +0.9 1,035,296 - BPHYT_RS24380 0.22 -0.0 1,035,575 - BPHYT_RS24380 0.43 +0.6 1,035,688 + BPHYT_RS24380 0.51 -0.2 1,035,789 - BPHYT_RS24380 0.59 +0.8 1,035,960 + BPHYT_RS24380 0.71 -0.9 1,035,964 + BPHYT_RS24380 0.72 +0.1
Or see this region's nucleotide sequence