Experiment: 36hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20705 and BPHYT_RS20710 are separated by 2 nucleotides BPHYT_RS20710 and BPHYT_RS20715 are separated by 2 nucleotides BPHYT_RS20715 and BPHYT_RS20720 are separated by 419 nucleotides BPHYT_RS20720 and BPHYT_RS20725 are separated by 18 nucleotides
BPHYT_RS20705: BPHYT_RS20705 - Chase2 sensor protein, at 206,098 to 207,810
_RS20705
BPHYT_RS20710: BPHYT_RS20710 - hypothetical protein, at 207,813 to 208,193
_RS20710
BPHYT_RS20715: BPHYT_RS20715 - peptidase, at 208,196 to 209,902
_RS20715
BPHYT_RS20720: BPHYT_RS20720 - hypothetical protein, at 210,322 to 210,588
_RS20720
BPHYT_RS20725: BPHYT_RS20725 - histidine kinase, at 210,607 to 211,944
_RS20725
Position (kb) 208
209
210 Strain fitness (log2 ratio) -2
-1
0
1 at 207.731 kb on + strand at 207.933 kb on + strand, within BPHYT_RS20710 at 207.934 kb on - strand, within BPHYT_RS20710 at 207.966 kb on + strand, within BPHYT_RS20710 at 208.242 kb on + strand at 209.370 kb on - strand, within BPHYT_RS20715 at 209.531 kb on - strand, within BPHYT_RS20715 at 210.439 kb on + strand, within BPHYT_RS20720 at 210.440 kb on - strand, within BPHYT_RS20720 at 210.574 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10 remove 207,731 + +0.4 207,933 + BPHYT_RS20710 0.31 +0.1 207,934 - BPHYT_RS20710 0.32 -0.2 207,966 + BPHYT_RS20710 0.40 +1.2 208,242 + +0.3 209,370 - BPHYT_RS20715 0.69 -0.9 209,531 - BPHYT_RS20715 0.78 -0.7 210,439 + BPHYT_RS20720 0.44 -1.1 210,440 - BPHYT_RS20720 0.44 -2.2 210,574 + +0.4
Or see this region's nucleotide sequence