Experiment: 36hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20045 and BPHYT_RS20050 are separated by 117 nucleotides BPHYT_RS20050 and BPHYT_RS20055 are separated by 342 nucleotides BPHYT_RS20055 and BPHYT_RS20060 are separated by 227 nucleotides BPHYT_RS20060 and BPHYT_RS20065 overlap by 1 nucleotides
BPHYT_RS20045: BPHYT_RS20045 - farnesyl-diphosphate farnesyltransferase, at 42,130 to 43,176
_RS20045
BPHYT_RS20050: BPHYT_RS20050 - hypothetical protein, at 43,294 to 43,542
_RS20050
BPHYT_RS20055: BPHYT_RS20055 - hypothetical protein, at 43,885 to 44,202
_RS20055
BPHYT_RS20060: BPHYT_RS20060 - hypothetical protein, at 44,430 to 45,122
_RS20060
BPHYT_RS20065: BPHYT_RS20065 - hypothetical protein, at 45,122 to 45,604
_RS20065
Position (kb) 43
44
45 Strain fitness (log2 ratio) -2
-1
0
1
2 at 43.219 kb on + strand at 43.242 kb on - strand at 43.312 kb on - strand at 43.776 kb on + strand at 43.843 kb on - strand at 43.886 kb on + strand at 44.069 kb on + strand, within BPHYT_RS20055 at 44.069 kb on + strand, within BPHYT_RS20055 at 44.236 kb on + strand at 44.360 kb on - strand at 44.639 kb on - strand, within BPHYT_RS20060 at 45.145 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10 remove 43,219 + -1.8 43,242 - -1.0 43,312 - -1.1 43,776 + +0.7 43,843 - -1.4 43,886 + -1.1 44,069 + BPHYT_RS20055 0.58 +1.8 44,069 + BPHYT_RS20055 0.58 -0.4 44,236 + +0.1 44,360 - +0.8 44,639 - BPHYT_RS20060 0.30 -1.3 45,145 + +2.1
Or see this region's nucleotide sequence