Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS10675

Experiment: 36hr PHYTO1 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS10670 and BPHYT_RS10675 are separated by 69 nucleotidesBPHYT_RS10675 and BPHYT_RS10680 are separated by 103 nucleotides BPHYT_RS10670: BPHYT_RS10670 - catechol 1,2-dioxygenase, at 2,429,520 to 2,430,428 _RS10670 BPHYT_RS10675: BPHYT_RS10675 - muconate cycloisomerase, at 2,430,498 to 2,431,655 _RS10675 BPHYT_RS10680: BPHYT_RS10680 - LysR family transcriptional regulator, at 2,431,759 to 2,432,646 _RS10680 Position (kb) 2430 2431 2432Strain fitness (log2 ratio) -1 0 1at 2429.780 kb on + strand, within BPHYT_RS10670at 2431.177 kb on + strand, within BPHYT_RS10675at 2431.755 kb on - strandat 2431.755 kb on - strandat 2432.524 kb on + strand, within BPHYT_RS10680at 2432.525 kb on - strand, within BPHYT_RS10680

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10
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2,429,780 + BPHYT_RS10670 0.29 -0.9
2,431,177 + BPHYT_RS10675 0.59 -1.0
2,431,755 - -0.1
2,431,755 - -1.1
2,432,524 + BPHYT_RS10680 0.86 -0.5
2,432,525 - BPHYT_RS10680 0.86 +0.7

Or see this region's nucleotide sequence