Experiment: 36hr PHYTO1 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09795 and BPHYT_RS09800 are separated by 361 nucleotides BPHYT_RS09800 and BPHYT_RS09805 are separated by 251 nucleotides BPHYT_RS09805 and BPHYT_RS09810 are separated by 5 nucleotides BPHYT_RS09810 and BPHYT_RS09815 are separated by 153 nucleotides
BPHYT_RS09795: BPHYT_RS09795 - UTP--glucose-1-phosphate uridylyltransferase, at 2,225,931 to 2,226,848
_RS09795
BPHYT_RS09800: BPHYT_RS09800 - hypothetical protein, at 2,227,210 to 2,227,542
_RS09800
BPHYT_RS09805: BPHYT_RS09805 - hypothetical protein, at 2,227,794 to 2,228,591
_RS09805
BPHYT_RS09810: BPHYT_RS09810 - hypothetical protein, at 2,228,597 to 2,228,821
_RS09810
BPHYT_RS09815: BPHYT_RS09815 - malate synthase, at 2,228,975 to 2,231,149
_RS09815
Position (kb)
2227
2228
2229 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2226.930 kb on + strand at 2227.030 kb on + strand at 2227.227 kb on - strand at 2227.347 kb on + strand, within BPHYT_RS09800 at 2227.541 kb on + strand at 2227.541 kb on + strand at 2227.624 kb on + strand at 2227.668 kb on + strand at 2227.789 kb on + strand at 2227.963 kb on - strand, within BPHYT_RS09805 at 2228.082 kb on + strand, within BPHYT_RS09805 at 2228.091 kb on + strand, within BPHYT_RS09805 at 2228.092 kb on - strand, within BPHYT_RS09805 at 2228.157 kb on + strand, within BPHYT_RS09805 at 2228.232 kb on + strand, within BPHYT_RS09805 at 2228.236 kb on - strand, within BPHYT_RS09805 at 2228.237 kb on + strand, within BPHYT_RS09805 at 2228.238 kb on - strand, within BPHYT_RS09805 at 2228.495 kb on - strand, within BPHYT_RS09805 at 2228.548 kb on - strand at 2228.548 kb on - strand at 2228.691 kb on - strand, within BPHYT_RS09810 at 2228.762 kb on + strand, within BPHYT_RS09810 at 2228.762 kb on + strand, within BPHYT_RS09810 at 2228.802 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO1 MOI 10 remove 2,226,930 + -0.2 2,227,030 + -0.1 2,227,227 - -1.1 2,227,347 + BPHYT_RS09800 0.41 +0.8 2,227,541 + -0.2 2,227,541 + -0.2 2,227,624 + -0.7 2,227,668 + +0.7 2,227,789 + +0.7 2,227,963 - BPHYT_RS09805 0.21 +0.1 2,228,082 + BPHYT_RS09805 0.36 +2.3 2,228,091 + BPHYT_RS09805 0.37 +1.1 2,228,092 - BPHYT_RS09805 0.37 -0.1 2,228,157 + BPHYT_RS09805 0.45 +0.5 2,228,232 + BPHYT_RS09805 0.55 +2.9 2,228,236 - BPHYT_RS09805 0.55 -0.3 2,228,237 + BPHYT_RS09805 0.56 -2.3 2,228,238 - BPHYT_RS09805 0.56 -0.6 2,228,495 - BPHYT_RS09805 0.88 +0.1 2,228,548 - +1.3 2,228,548 - -0.2 2,228,691 - BPHYT_RS09810 0.42 +1.3 2,228,762 + BPHYT_RS09810 0.73 +0.1 2,228,762 + BPHYT_RS09810 0.73 +0.9 2,228,802 - +0.8
Or see this region's nucleotide sequence