Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS34040

Experiment: 36hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS34035 and BPHYT_RS34040 are separated by 21 nucleotidesBPHYT_RS34040 and BPHYT_RS34045 are separated by 10 nucleotidesBPHYT_RS34045 and BPHYT_RS34050 are separated by 3 nucleotides BPHYT_RS34035: BPHYT_RS34035 - type II and III secretion system outermembrane protein, secretin, at 3,235,195 to 3,236,880 _RS34035 BPHYT_RS34040: BPHYT_RS34040 - hypothetical protein, at 3,236,902 to 3,238,806 _RS34040 BPHYT_RS34045: BPHYT_RS34045 - secretion system protein F, at 3,238,817 to 3,239,665 _RS34045 BPHYT_RS34050: BPHYT_RS34050 - secretion system protein F, at 3,239,669 to 3,240,550 _RS34050 Position (kb) 3236 3237 3238 3239Strain fitness (log2 ratio) -1 0 1 2at 3236.157 kb on + strand, within BPHYT_RS34035at 3236.235 kb on + strand, within BPHYT_RS34035at 3236.999 kb on + strandat 3236.999 kb on + strandat 3237.000 kb on - strandat 3237.003 kb on + strandat 3237.061 kb on + strandat 3237.062 kb on - strandat 3237.411 kb on + strand, within BPHYT_RS34040at 3237.412 kb on - strand, within BPHYT_RS34040at 3238.018 kb on - strand, within BPHYT_RS34040at 3238.728 kb on + strandat 3238.728 kb on + strandat 3238.729 kb on - strandat 3238.742 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only
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3,236,157 + BPHYT_RS34035 0.57 +1.8
3,236,235 + BPHYT_RS34035 0.62 +0.2
3,236,999 + -0.0
3,236,999 + -0.4
3,237,000 - +1.9
3,237,003 + +0.3
3,237,061 + +1.2
3,237,062 - -0.2
3,237,411 + BPHYT_RS34040 0.27 +2.0
3,237,412 - BPHYT_RS34040 0.27 -0.3
3,238,018 - BPHYT_RS34040 0.59 +0.0
3,238,728 + +0.2
3,238,728 + -1.7
3,238,729 - +1.2
3,238,742 - -0.5

Or see this region's nucleotide sequence