Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS33360

Experiment: 36hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS33355 and BPHYT_RS33360 are separated by 24 nucleotidesBPHYT_RS33360 and BPHYT_RS33365 are separated by 105 nucleotidesBPHYT_RS33365 and BPHYT_RS33370 are separated by 80 nucleotides BPHYT_RS33355: BPHYT_RS33355 - oxalyl-CoA decarboxylase, at 3,066,064 to 3,067,806 _RS33355 BPHYT_RS33360: BPHYT_RS33360 - formyl-CoA transferase, at 3,067,831 to 3,069,081 _RS33360 BPHYT_RS33365: BPHYT_RS33365 - PAS sensor protein, at 3,069,187 to 3,069,618 _RS33365 BPHYT_RS33370: BPHYT_RS33370 - LysR family transcriptional regulator, at 3,069,699 to 3,070,625 _RS33370 Position (kb) 3067 3068 3069 3070Strain fitness (log2 ratio) -1 0 1at 3067.538 kb on + strand, within BPHYT_RS33355at 3067.539 kb on - strand, within BPHYT_RS33355at 3068.228 kb on - strand, within BPHYT_RS33360at 3068.519 kb on - strand, within BPHYT_RS33360at 3068.630 kb on - strand, within BPHYT_RS33360at 3069.089 kb on + strandat 3069.327 kb on + strand, within BPHYT_RS33365at 3069.450 kb on + strand, within BPHYT_RS33365at 3069.892 kb on - strand, within BPHYT_RS33370

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only
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3,067,538 + BPHYT_RS33355 0.85 -1.2
3,067,539 - BPHYT_RS33355 0.85 -0.2
3,068,228 - BPHYT_RS33360 0.32 -0.2
3,068,519 - BPHYT_RS33360 0.55 -1.2
3,068,630 - BPHYT_RS33360 0.64 -1.3
3,069,089 + -1.3
3,069,327 + BPHYT_RS33365 0.32 -0.2
3,069,450 + BPHYT_RS33365 0.61 -1.1
3,069,892 - BPHYT_RS33370 0.21 +1.0

Or see this region's nucleotide sequence