Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22765

Experiment: 36hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22760 and BPHYT_RS22765 are separated by 11 nucleotidesBPHYT_RS22765 and BPHYT_RS22770 are separated by 89 nucleotides BPHYT_RS22760: BPHYT_RS22760 - sugar ABC transporter ATP-binding protein, at 663,638 to 664,792 _RS22760 BPHYT_RS22765: BPHYT_RS22765 - beta-galactosidase, at 664,804 to 666,786 _RS22765 BPHYT_RS22770: BPHYT_RS22770 - sugar ABC transporter substrate-binding protein, at 666,876 to 668,129 _RS22770 Position (kb) 664 665 666 667Strain fitness (log2 ratio) -1 0 1at 664.328 kb on + strand, within BPHYT_RS22760at 664.619 kb on + strand, within BPHYT_RS22760at 664.620 kb on - strand, within BPHYT_RS22760at 664.620 kb on - strand, within BPHYT_RS22760at 664.822 kb on + strandat 665.704 kb on + strand, within BPHYT_RS22765at 666.112 kb on + strand, within BPHYT_RS22765at 666.112 kb on + strand, within BPHYT_RS22765at 666.179 kb on - strand, within BPHYT_RS22765at 666.180 kb on + strand, within BPHYT_RS22765at 666.775 kb on + strandat 667.240 kb on - strand, within BPHYT_RS22770at 667.240 kb on - strand, within BPHYT_RS22770at 667.240 kb on - strand, within BPHYT_RS22770at 667.564 kb on - strand, within BPHYT_RS22770

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only
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664,328 + BPHYT_RS22760 0.60 +0.5
664,619 + BPHYT_RS22760 0.85 -0.7
664,620 - BPHYT_RS22760 0.85 +0.3
664,620 - BPHYT_RS22760 0.85 +1.6
664,822 + -0.4
665,704 + BPHYT_RS22765 0.45 -1.1
666,112 + BPHYT_RS22765 0.66 -0.1
666,112 + BPHYT_RS22765 0.66 -0.9
666,179 - BPHYT_RS22765 0.69 -1.1
666,180 + BPHYT_RS22765 0.69 +0.1
666,775 + -0.4
667,240 - BPHYT_RS22770 0.29 -1.3
667,240 - BPHYT_RS22770 0.29 +0.3
667,240 - BPHYT_RS22770 0.29 -0.8
667,564 - BPHYT_RS22770 0.55 -0.1

Or see this region's nucleotide sequence