Experiment: 36hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21470 and BPHYT_RS21475 are separated by 52 nucleotides BPHYT_RS21475 and BPHYT_RS21480 overlap by 25 nucleotides BPHYT_RS21480 and BPHYT_RS21485 are separated by 73 nucleotides
BPHYT_RS21470: BPHYT_RS21470 - 6-hydroxynicotinate reductase, at 376,702 to 378,306
_RS21470
BPHYT_RS21475: BPHYT_RS21475 - hypothetical protein, at 378,359 to 379,297
_RS21475
BPHYT_RS21480: BPHYT_RS21480 - hypothetical protein, at 379,273 to 379,863
_RS21480
BPHYT_RS21485: BPHYT_RS21485 - hypothetical protein, at 379,937 to 380,521
_RS21485
Position (kb) 378
379
380 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 378.022 kb on + strand, within BPHYT_RS21470 at 378.219 kb on + strand at 378.246 kb on + strand at 378.598 kb on + strand, within BPHYT_RS21475 at 378.599 kb on - strand, within BPHYT_RS21475 at 378.599 kb on - strand, within BPHYT_RS21475 at 378.632 kb on + strand, within BPHYT_RS21475 at 378.633 kb on - strand, within BPHYT_RS21475 at 378.746 kb on + strand, within BPHYT_RS21475 at 379.771 kb on + strand, within BPHYT_RS21480 at 379.772 kb on - strand, within BPHYT_RS21480 at 379.772 kb on - strand, within BPHYT_RS21480 at 379.774 kb on - strand, within BPHYT_RS21480 at 380.048 kb on - strand, within BPHYT_RS21485
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only remove 378,022 + BPHYT_RS21470 0.82 -0.3 378,219 + -0.2 378,246 + +0.6 378,598 + BPHYT_RS21475 0.25 +1.4 378,599 - BPHYT_RS21475 0.26 -1.8 378,599 - BPHYT_RS21475 0.26 -0.1 378,632 + BPHYT_RS21475 0.29 +0.1 378,633 - BPHYT_RS21475 0.29 +0.1 378,746 + BPHYT_RS21475 0.41 +3.4 379,771 + BPHYT_RS21480 0.84 -0.8 379,772 - BPHYT_RS21480 0.84 +0.3 379,772 - BPHYT_RS21480 0.84 -0.8 379,774 - BPHYT_RS21480 0.85 +0.5 380,048 - BPHYT_RS21485 0.19 -1.3
Or see this region's nucleotide sequence