Experiment: 36hr Phyto Lib Only
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20105 and BPHYT_RS20110 are separated by 66 nucleotides BPHYT_RS20110 and BPHYT_RS20115 are separated by 25 nucleotides BPHYT_RS20115 and BPHYT_RS20120 are separated by 182 nucleotides
BPHYT_RS20105: BPHYT_RS20105 - nitrate ABC transporter substrate-binding protein, at 53,379 to 54,419
_RS20105
BPHYT_RS20110: BPHYT_RS20110 - sensor histidine kinase, at 54,486 to 55,868
_RS20110
BPHYT_RS20115: BPHYT_RS20115 - transcriptional regulator, at 55,894 to 56,592
_RS20115
BPHYT_RS20120: BPHYT_RS20120 - porin, at 56,775 to 57,899
_RS20120
Position (kb) 54
55
56 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 53.895 kb on + strand, within BPHYT_RS20105 at 53.896 kb on - strand, within BPHYT_RS20105 at 54.035 kb on + strand, within BPHYT_RS20105 at 54.036 kb on - strand, within BPHYT_RS20105 at 54.421 kb on - strand at 54.738 kb on + strand, within BPHYT_RS20110 at 54.979 kb on + strand, within BPHYT_RS20110 at 55.023 kb on + strand, within BPHYT_RS20110 at 55.628 kb on + strand, within BPHYT_RS20110 at 55.877 kb on + strand at 56.026 kb on + strand, within BPHYT_RS20115 at 56.469 kb on + strand, within BPHYT_RS20115 at 56.475 kb on + strand, within BPHYT_RS20115 at 56.625 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only remove 53,895 + BPHYT_RS20105 0.50 +2.2 53,896 - BPHYT_RS20105 0.50 -2.7 54,035 + BPHYT_RS20105 0.63 +0.6 54,036 - BPHYT_RS20105 0.63 -0.4 54,421 - -0.1 54,738 + BPHYT_RS20110 0.18 +1.2 54,979 + BPHYT_RS20110 0.36 +0.9 55,023 + BPHYT_RS20110 0.39 +0.2 55,628 + BPHYT_RS20110 0.83 -0.2 55,877 + -2.2 56,026 + BPHYT_RS20115 0.19 +3.1 56,469 + BPHYT_RS20115 0.82 +0.5 56,475 + BPHYT_RS20115 0.83 +0.4 56,625 - -2.7
Or see this region's nucleotide sequence