Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS16285

Experiment: 36hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS16275 and BPHYT_RS16280 overlap by 4 nucleotidesBPHYT_RS16280 and BPHYT_RS16285 are separated by 220 nucleotidesBPHYT_RS16285 and BPHYT_RS16290 are separated by 62 nucleotidesBPHYT_RS16290 and BPHYT_RS16295 are separated by 169 nucleotides BPHYT_RS16275: BPHYT_RS16275 - branched-chain amino acid transporter, at 3,695,852 to 3,696,175 _RS16275 BPHYT_RS16280: BPHYT_RS16280 - branched-chain amino acid ABC transporter permease, at 3,696,172 to 3,696,918 _RS16280 BPHYT_RS16285: BPHYT_RS16285 - Branched-chain amino acid aminotransferase (EC 2.6.1.42) (from data), at 3,697,139 to 3,698,062 _RS16285 BPHYT_RS16290: BPHYT_RS16290 - hypothetical protein, at 3,698,125 to 3,698,319 _RS16290 BPHYT_RS16295: BPHYT_RS16295 - ADP-heptose--LPS heptosyltransferase, at 3,698,489 to 3,699,514 _RS16295 Position (kb) 3697 3698 3699Strain fitness (log2 ratio) -1 0 1 2 3at 3696.421 kb on - strand, within BPHYT_RS16280at 3696.503 kb on + strand, within BPHYT_RS16280at 3696.504 kb on - strand, within BPHYT_RS16280at 3696.506 kb on + strand, within BPHYT_RS16280at 3696.506 kb on + strand, within BPHYT_RS16280at 3696.507 kb on - strand, within BPHYT_RS16280at 3696.507 kb on - strand, within BPHYT_RS16280at 3696.507 kb on - strand, within BPHYT_RS16280at 3696.507 kb on - strand, within BPHYT_RS16280at 3696.507 kb on - strand, within BPHYT_RS16280at 3696.507 kb on - strand, within BPHYT_RS16280at 3696.527 kb on + strand, within BPHYT_RS16280at 3696.527 kb on + strand, within BPHYT_RS16280at 3696.527 kb on + strand, within BPHYT_RS16280at 3696.563 kb on + strand, within BPHYT_RS16280at 3696.564 kb on - strand, within BPHYT_RS16280at 3696.564 kb on - strand, within BPHYT_RS16280at 3696.564 kb on - strand, within BPHYT_RS16280at 3696.564 kb on - strand, within BPHYT_RS16280at 3696.618 kb on - strand, within BPHYT_RS16280at 3696.663 kb on + strand, within BPHYT_RS16280at 3696.753 kb on - strand, within BPHYT_RS16280at 3696.897 kb on + strandat 3696.897 kb on + strandat 3696.898 kb on - strandat 3696.898 kb on - strandat 3697.009 kb on + strandat 3697.009 kb on + strandat 3697.010 kb on - strandat 3697.026 kb on - strandat 3697.027 kb on + strandat 3697.028 kb on - strandat 3697.074 kb on + strandat 3697.093 kb on - strandat 3697.094 kb on + strandat 3697.103 kb on + strandat 3697.103 kb on + strandat 3697.103 kb on + strandat 3697.107 kb on + strandat 3697.107 kb on + strandat 3697.107 kb on + strandat 3697.113 kb on + strandat 3697.138 kb on + strandat 3697.138 kb on + strandat 3697.430 kb on + strand, within BPHYT_RS16285at 3697.430 kb on + strand, within BPHYT_RS16285at 3697.431 kb on - strand, within BPHYT_RS16285at 3697.529 kb on + strand, within BPHYT_RS16285at 3697.529 kb on + strand, within BPHYT_RS16285at 3697.880 kb on + strand, within BPHYT_RS16285at 3697.880 kb on + strand, within BPHYT_RS16285at 3698.108 kb on + strandat 3698.108 kb on + strandat 3698.281 kb on + strandat 3698.281 kb on + strand, within BPHYT_RS16290at 3698.387 kb on + strandat 3698.416 kb on + strandat 3698.444 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only
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3,696,421 - BPHYT_RS16280 0.33 +0.3
3,696,503 + BPHYT_RS16280 0.44 -0.2
3,696,504 - BPHYT_RS16280 0.44 -0.0
3,696,506 + BPHYT_RS16280 0.45 -0.2
3,696,506 + BPHYT_RS16280 0.45 +0.1
3,696,507 - BPHYT_RS16280 0.45 -0.0
3,696,507 - BPHYT_RS16280 0.45 -0.3
3,696,507 - BPHYT_RS16280 0.45 +0.4
3,696,507 - BPHYT_RS16280 0.45 +0.5
3,696,507 - BPHYT_RS16280 0.45 -0.0
3,696,507 - BPHYT_RS16280 0.45 -0.1
3,696,527 + BPHYT_RS16280 0.48 +1.0
3,696,527 + BPHYT_RS16280 0.48 -0.2
3,696,527 + BPHYT_RS16280 0.48 -0.4
3,696,563 + BPHYT_RS16280 0.52 -0.2
3,696,564 - BPHYT_RS16280 0.52 +0.9
3,696,564 - BPHYT_RS16280 0.52 -0.6
3,696,564 - BPHYT_RS16280 0.52 -0.9
3,696,564 - BPHYT_RS16280 0.52 -1.6
3,696,618 - BPHYT_RS16280 0.60 -0.5
3,696,663 + BPHYT_RS16280 0.66 -0.3
3,696,753 - BPHYT_RS16280 0.78 -0.3
3,696,897 + -0.3
3,696,897 + -0.3
3,696,898 - -0.2
3,696,898 - -0.7
3,697,009 + -0.3
3,697,009 + -0.9
3,697,010 - -1.5
3,697,026 - -0.4
3,697,027 + -0.5
3,697,028 - -0.3
3,697,074 + +0.5
3,697,093 - +0.5
3,697,094 + +0.1
3,697,103 + +0.3
3,697,103 + -0.6
3,697,103 + -0.1
3,697,107 + -0.0
3,697,107 + -0.4
3,697,107 + +1.1
3,697,113 + -0.7
3,697,138 + -0.8
3,697,138 + -0.4
3,697,430 + BPHYT_RS16285 0.31 +3.4
3,697,430 + BPHYT_RS16285 0.31 -0.5
3,697,431 - BPHYT_RS16285 0.32 +1.8
3,697,529 + BPHYT_RS16285 0.42 +0.0
3,697,529 + BPHYT_RS16285 0.42 +0.3
3,697,880 + BPHYT_RS16285 0.80 +0.6
3,697,880 + BPHYT_RS16285 0.80 +0.2
3,698,108 + +0.2
3,698,108 + +0.1
3,698,281 + -1.3
3,698,281 + BPHYT_RS16290 0.80 -0.0
3,698,387 + -0.5
3,698,416 + -0.5
3,698,444 + +0.8

Or see this region's nucleotide sequence