Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05930

Experiment: 36hr Phyto Lib Only

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05925 and BPHYT_RS05930 are separated by 356 nucleotidesBPHYT_RS05930 and BPHYT_RS05935 are separated by 80 nucleotides BPHYT_RS05925: BPHYT_RS05925 - ABC transporter ATP-binding protein, at 1,343,661 to 1,345,607 _RS05925 BPHYT_RS05930: BPHYT_RS05930 - DNA topoisomerase IV subunit B, at 1,345,964 to 1,347,949 _RS05930 BPHYT_RS05935: BPHYT_RS05935 - DNA topoisomerase IV subunit A, at 1,348,030 to 1,350,354 _RS05935 Position (kb) 1345 1346 1347 1348Strain fitness (log2 ratio) -1 0 1at 1345.010 kb on + strand, within BPHYT_RS05925at 1345.228 kb on - strand, within BPHYT_RS05925at 1345.706 kb on - strandat 1345.710 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr Phyto Lib Only
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1,345,010 + BPHYT_RS05925 0.69 +0.5
1,345,228 - BPHYT_RS05925 0.80 -0.7
1,345,706 - -0.1
1,345,710 + +0.1

Or see this region's nucleotide sequence